ArticleThe Biochemical journal1990
Regulation of procollagen synthesis and processing during ascorbate-induced extracellular matrix accumulation in vitro.
Article in The Biochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 125 citations in OpenAlex.
- 3-O-ethyl-L-ascorbic acid modulates pro-inflammatory cytokine and extracellular matrix-related biomarker levels in human lung fibroblasts: anActa biochimica Polonica · 2026Article
- Modulation of Canine Adipose-Derived Mesenchymal Stem/Medicinal Signalling Cells with Ascorbic Acid: Effect on Proliferation and Chondrogenic Differentiation on Standard Plastic and Silk Fibroin Surfaces.Bioengineering (Basel, Switzerland) · 2024Article
- Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.JBMR plus · 2023Article
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- Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts.Biomaterials science · 2022Article
- In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model.Materials (Basel, Switzerland) · 2021Article
- The Human Tissue-Engineered Cornea (hTEC): Recent Progress.International journal of molecular sciences · 2021Review
- The Enhanced Adhesion of Eosinophils Is Associated with Their Prolonged Viability and Pro-Proliferative Effect in Asthma.Journal of clinical medicine · 2019Article
- A novel human 3D lung microtissue model for nanoparticle-induced cell-matrix alterations.Particle and fibre toxicology · 2019Article
- Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells.Stem cell research & therapy · 2018Article
- The epigenetic role of vitamin C in health and disease.Cellular and molecular life sciences : CMLS · 2016Review
- The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro.Cellular & molecular biology letters · 2016Article
- Production of ascorbic acid releasing biomaterials for pelvic floor repair.Acta biomaterialia · 2016Article
- Protease inhibitors enhance extracellular collagen fibril deposition in human mesenchymal stem cells.Stem cell research & therapy · 2015Article
- Structural basis for misfolding in myocilin-associated glaucoma.Human molecular genetics · 2015Article
- Collagen V localizes to pericellular sites during tendon collagen fibrillogenesis.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Dietary flounder skin improves growth performance, body composition, and stress recovery in the juvenile black rockfish (Sebastes schlegeli).SpringerPlus · 2014Article
- Collagen-cellulose composite thin films that mimic soft-tissue and allow stem-cell orientation.Journal of materials science. Materials in medicine · 2013Article
- An in vitro study of osteoblast vitality influenced by the vitamins C and E.Head & face medicine · 2012Article
- Regulation of polysome assembly on the endoplasmic reticulum by a coiled-coil protein, p180.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Procollagen biosynthesis and matrix deposition were studied in long-term human skin fibroblast cultures exposed to ascorbic acid. Ascorbic acid specifically stimulated types I and III collagen synthesis, reaching a maximum at day 2 and maintaining a specific high rate of production until day 10 of ascorbate exposure, after which collagen production declined. The increased level of collagen synthesis after different exposure times could also be achieved by only brief treatment (10 h) of parallel scorbutic (ascorbic-acid-deficient) cultures with ascorbic acid. This brief exposure did not result in increased collagen mRNA, thus demonstrating that the ascorbate-induced increase in collagen synthesis at all stages of ascorbic acid exposure was due to post-transcriptional mechanisms, most likely a rapid increase in type 1 collagen mRNA translational efficiency. This mechanism, rather than the transcriptional activation, was the primary response and is adequate to explain the ascorbate-induced increase in collagen synthesis. These data also demonstrate that the presence of a collagenous extracellular matrix was not involved in this collagen biosynthetic regulation. During long-term exposure (18 days) to ascorbic acid, a substantial cross-linked collagenous matrix formed, following an approximately sigmoidal time course. The most rapid matrix deposition occurred during the later days of exposure when the rate of collagen synthesis was decreasing, suggesting that the presence of a pre-existing matrix is important for further collagen accumulation. Procollagen was also efficiently processed to collagen during this phase, demonstrating that efficient procollagen processing is an important regulatory event in collagen matrix deposition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.