ArticleProceedings of the National Academy of Sciences of the United States of America2013
Polyester synthesis genes associated with stress resistance are involved in an insect-bacterium symbiosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 94 citations in OpenAlex.
- InferiorMicrobial genomics · 2024Pooled it
- Coupling Nitrogenous Organic Wastewater Treatment and Biorefinery via N-Cycling Bacterium.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Bacteria break through one-micrometer-square passages by flagellar wrapping.Nature communications · 2026Article
- Temperature influences outcomes of an environmentally acquired symbiosis.The ISME journal · 2025Article
- BglaTNB6, a tailocin produced by a plant-associated nonpathogenic bacterium, prevents rice seed-borne bacterial diseases.PLoS pathogens · 2024Article
- The plant-sucking insect selects assembly of the gut microbiota from environment to enhance host reproduction.NPJ biofilms and microbiomes · 2024Article
- Polyhydroxyalkanoate involvement in stress-survival of two psychrophilic bacterial strains from the High Arctic.Applied microbiology and biotechnology · 2024Article
- Specialized digestive mechanism for an insect-bacterium gut symbiosis.The ISME journal · 2024Article
- Proteolytic Activity of DegP Is Required for theMicrobiology spectrum · 2023Article
- Alteration of lipopolysaccharide O antigen leads to avirulence of gut-colonizingFrontiers in microbiology · 2023Article
- Prevalence of an Insect-Associated Genomic Region in Environmentally AcquiredApplied and environmental microbiology · 2022Article
- Insecticidal Serralysin ofFrontiers in microbiology · 2022Article
- Insecticide resistance by a host-symbiont reciprocal detoxification.Nature communications · 2021Article
- Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces.mSystems · 2021Article
- Insecticide resistance governed by gut symbiosis in a rice pest,Biology letters · 2021Article
- The Gut Microbiota of the Insect Infraorder Pentatomomorpha (Hemiptera: Heteroptera) for the Light of Ecology and Evolution.Microorganisms · 2021Review
- The Importance of Environmentally Acquired Bacterial Symbionts for the Squash Bug (Frontiers in microbiology · 2021Article
- Multiple Precursor Proteins of Thanatin Isoforms, an Antimicrobial Peptide Associated With the Gut Symbiont ofFrontiers in microbiology · 2021Article
- 3-Hydroxybutyrate Derived from Poly-3-Hydroxybutyrate Mobilization Alleviates Protein Aggregation in Heat-Stressed Herbaspirillum seropedicae SmR1.Applied and environmental microbiology · 2020Article
- Article
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bacteria accumulate granules of polyhydroxyalkanoate (PHA) within their cells, which confer resistance to nutritional depletion and other environmental stresses. Here, we report an unexpected involvement of the bacterial endocellular storage polymer, PHA, in an insect-bacterium symbiotic association. The bean bug Riptortus pedestris harbors a beneficial and specific gut symbiont of the β-proteobacterial genus Burkholderia, which is orally acquired by host nymphs from the environment every generation and easily cultivable and genetically manipulatable. Biochemical and cytological comparisons between symbiotic and cultured Burkholderia detected more PHA granules consisting of poly-3-hydroxybutyrate and associated phasin (PhaP) protein in the symbiotic Burkholderia. Among major PHA synthesis genes, phaB and phaC were disrupted by homologous recombination together with the phaP gene, whereby ΔphaB, ΔphaC, and ΔphaP mutants were generated. Both in culture and in symbiosis, accumulation of PHA granules was strongly suppressed in ΔphaB and ΔphaC, but only moderately in ΔphaP. In symbiosis, the host insects infected with ΔphaB and ΔphaC exhibited significantly lower symbiont densities and smaller body sizes. These deficient phenotypes associated with ΔphaB and ΔphaC were restored by complementation of the mutants with plasmids encoding a functional phaB/phaC gene. Retention analysis of the plasmids revealed positive selection acting on the functional phaB/phaC in symbiosis. These results indicate that the PHA synthesis genes of the Burkholderia symbiont are required for normal symbiotic association with the Riptortus host. In vitro culturing analyses confirmed vulnerability of the PHA gene mutants to environmental stresses, suggesting that PHA may play a role in resisting stress under symbiotic conditions.
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