Evidence map›Paper›PMID 23757305›Full record

ArticleJPEN. Journal of parenteral and enteral nutrition2014

Ω-3 fatty acids prevent hepatic steatosis, independent of PPAR-α activity, in a murine model of parenteral nutrition-associated liver disease.

Esther Prince, Farrah B Lazare, William R Treem, Jiliu Xu, Jahangir Iqbal, Xiaoyue Pan, Joby Josekutty, Meghan Walsh, Virginia Anderson, M Mahmood Hussain and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JPEN. Journal of parenteral and enteral nutrition, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Hepatoprotective and Antioxidant Activities of Oil from Baru Almonds (Evidence-based complementary and alternative medicine : eCAM · 2018
    Article
  6. [Research advances in liver diseases associated with parenteral nutrition].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2017
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Esther PrinceDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, State University of New York Downstate Medical Center, Brooklyn, New York.
Farrah B LazareDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, State University of New York Downstate Medical Center, Brooklyn, New York Department of Pediatrics, Winthrop University Medical Center, Mineola, New York.
William R TreemDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, State University of New York Downstate Medical Center, Brooklyn, New York Johnson & Johnson Pharmaceutical Research and Development, LLC, Titusville, New Jersey.
Jiliu XuDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, State University of New York Downstate Medical Center, Brooklyn, New York.
Jahangir IqbalDepartment of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York.
Xiaoyue PanDepartment of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York.
Joby JosekuttyDepartment of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York.
Meghan WalshDepartment of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York.
Virginia AndersonDepartment of Pathology, State University of New York Downstate Medical Center, Brooklyn, New York.
M Mahmood HussainDepartment of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York.
Steven M SchwarzDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, State University of New York Downstate Medical Center, Brooklyn, New York steven.schwarz@downstate.edu.
SUNY Downstate Health Sciences University · USState University of New York · USJohnson & Johnson (United States) · USUniversity Medical Center · US

Funding

MOLECULAR MECHANISMS OF CHYLOMICRON ASSEMBLYR01DK046900 · NIDDK · SUNY DOWNSTATE MEDICAL CENTER · PI HUSSAIN, M MAHMOOD · 2001 to 2011
$3.0M
Diurnal regulation of MTP and plasma lipidsR01DK081879 · NIDDK · SUNY DOWNSTATE MEDICAL CENTER · PI HUSSAIN, M MAHMOOD · 2009 to 2014
$2.3M
Avoiding toxicity associated with MTP ablationR01HL095924 · NHLBI · SUNY DOWNSTATE MEDICAL CENTER · PI HUSSAIN, M MAHMOOD · 2010 to 2013
$1.9M
NHLBI NIH HHS HL-095924NHLBI NIH HHS R01 HL095924NIDDK NIH HHS DK-46700NIDDK NIH HHS R01 DK046900NIDDK NIH HHS R01 DK081879
6 · The paper itself

Abstract

objectivesω-3 Fatty acids (FAs), natural ligands for the peroxisome proliferator-activated receptor-α (PPAR-α), attenuate parenteral nutrition-associated liver disease (PNALD). However, the mechanisms underlying the protective role of ω-3 FAs are still unknown. The aim of this study was to determine the effects of ω-3 FAs on hepatic triglyceride (TG) accumulation in a murine model of PNALD and to investigate the role of PPAR-α and microsomal triglyceride transfer protein (MTP) in this experimental setting.

methods129S1/SvImJ wild-type or 129S4/SvJaePparatm/Gonz/J PPAR-α knockout mice were fed chow and water (controls); oral, fat-free PN solution only (PN-O); PN-O plus intraperitoneal (IP) ω-6 FA-predominant supplements (PN-ω-6); or PN-O plus IP ω-3 FA (PN-ω-3). Control and PN-O groups received sham IP injections of 0.9% NaCl. Hepatic histology, TG and cholesterol, MTP activity, and PPAR-α messenger RNA were assessed after 19 days.

resultsIn all experimental groups, PN feeding increased hepatic TG and MTP activity compared with controls. Both PN-O and PN-ω-6 groups accumulated significantly greater amounts of TG when compared with PN-ω-3 mice. Studies in PPAR-α null animals showed that PN feeding increases hepatic TG as in wild-type mice. PPAR-α null mice in the PN-O and PN-ω-6 groups demonstrated variable degrees of hepatic steatosis, whereas no evidence of hepatic fat accumulation was found after 19 days of oral PN plus IP ω-3 FAs.

conclusionsPN induces TG accumulation (steatosis) in wild-type and PPAR-α null mice. In PN-fed wild-type and PPAR-α null mice given IP ω-3 FAs, reduced hepatic TG accumulation and absent steatosis are found. Prevention of steatosis by ω-3 FAs results from PPAR-α-independent pathways.

Indexed as

Parenteral NutritionAnimalsCarrier ProteinsCholesterolDisease Models, AnimalFatty Acids, Omega-3Fatty LiverLiverMaleMiceMice, Inbred StrainsMice, KnockoutPPAR alphaTreatment OutcomeTriglyceridesCarrier ProteinsCholesterolFatty Acids, Omega-3microsomal triglyceride transfer proteinPPAR alphaTriglyceridesmicrosomal triglyceride transfer proteinparenteral nutrition–associated liver diseaseperoxisome proliferator-activated receptor–αsteatosisω-3-fatty acids

Identifiers

PMID23757305
PMCPMC4372508
OpenAlexW2091428788

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.