ArticleEnvironmental science & technology2013
Performance characteristics of qPCR assays targeting human- and ruminant-associated bacteroidetes for microbial source tracking across sixteen countries on six continents.
Article in Environmental science & technology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 2 of them syntheses that pooled it.
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Who cites it
49 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Is detection of enteropathogens and human or animal faecal markers in the environment associated with subsequent child enteric infections and growth: an individual participant data meta-analysis.The Lancet. Global health · 2024Pooled it
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- The probability of detecting host-specific microbial source tracking markers in surface waters was strongly associated with method and season.Microbiology spectrum · 2025Article
- Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, andHeliyon · 2024Article
- Correlations among Antibiotic Resistance Genes, Mobile Genetic Elements and Microbial Communities in Municipal Sewage Treatment Plants Revealed by High-Throughput Sequencing.International journal of environmental research and public health · 2023Article
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Article
- Genetic sequence data evidence that human faecal-associated HF183 sequences are on human skin and in urine.Journal of applied microbiology · 2022Article
- Elevated Fecal Mitochondrial DNA from Symptomatic Norovirus Infections Suggests Potential Health Relevance of Human Mitochondrial DNA in Fecal Source Tracking.Environmental science & technology letters · 2022Article
- Human Fecal Contamination Corresponds to Changes in the Freshwater Bacterial Communities of a Large River Basin.Microbiology spectrum · 2021Article
- Assessing the faecal source sensitivity and specificity of ruminant and human genetic microbial source tracking markers in the central Ethiopian highlands.Letters in applied microbiology · 2021Article
- Microbial source tracking using metagenomics and other new technologies.Journal of microbiology (Seoul, Korea) · 2021Review
- Synergistic Application of Molecular Markers and Community-Based Microbial Source Tracking Methods for Identification of Fecal Pollution in River Water During Dry and Wet Seasons.Frontiers in microbiology · 2021Article
- Identifying Inorganic Turbidity in Water Samples as Potential Loss Factor During Nucleic Acid Extraction: Implications for Molecular Fecal Pollution Diagnostics and Source Tracking.Frontiers in microbiology · 2021Article
- Comparative Analysis of Fecal Microbiomes From Wild Waterbirds to Poultry, Cattle, Pigs, and Wastewater Treatment Plants for a Microbial Source Tracking Approach.Frontiers in microbiology · 2021Article
- Genetic Microbial Source Tracking Support QMRA Modeling for a Riverine Wetland Drinking Water Resource.Frontiers in microbiology · 2021Article
- Evaluation of Low-Cost Phage-Based Microbial Source Tracking Tools for Elucidating Human Fecal Contamination Pathways in Kolkata, India.Frontiers in microbiology · 2021Article
- Shared bacterial communities between soil, stored drinking water, and hands in rural Bangladeshi households.Water research X · 2020Article
- A novel droplet digital PCR human mtDNA assay for fecal source tracking.Water research · 2020Article
- Human fecal contamination of water, soil, and surfaces in households sharing poor-quality sanitation facilities in Maputo, Mozambique.International journal of hygiene and environmental health · 2020Article
Corrections and comments
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Authors and funding
26 authors.
Funding
Abstract
Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle-, or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and nontarget samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant-targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable, suggesting potential for improvements in human-targeted methods.
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