ArticlePloS one2013
High resolution imaging of temporal and spatial changes of subcellular ascorbate, glutathione and H₂O₂ distribution during Botrytis cinerea infection in Arabidopsis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Sulfur as a central integrator of plant-microbe interactions: from nutrient cycling to immune signalling and microbiome assembly.Journal of experimental botany · 2026Review
- Advances in the Application of Amino Acids in the Preservation of Postharvest Fruits and Vegetables.Foods (Basel, Switzerland) · 2026Review
- Multiomics analysis of cassava with different ploidy levels in response toTetranychus cinnabarinus.BMC plant biology · 2025Article
- Superoxide signalling and antioxidant processing in the plant nucleus.Journal of experimental botany · 2024Review
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- Overexpression of Sly-miR398b Compromises Disease Resistance againstPlants (Basel, Switzerland) · 2023Article
- AtGSTU19 and AtGSTU24 as Moderators of the Response ofInternational journal of molecular sciences · 2022Article
- Conserved secreted effectors contribute to endophytic growth and multihost plant compatibility in a vascular wilt fungus.The Plant cell · 2022Article
- Glutathione Modulation in PVYInternational journal of molecular sciences · 2022Article
- Three-dimensional quantitative imaging of Tobacco mosaic virus and Zucchini yellow mosaic virus induced ultrastructural changes.Protoplasma · 2021Review
- Fructans Prime ROS Dynamics andAntioxidants (Basel, Switzerland) · 2020Article
- Subcellular Roles of Glutathione in Mediating Plant Defense during Biotic Stress.Plants (Basel, Switzerland) · 2020Review
- Comparative genomics of Alternaria species provides insights into the pathogenic lifestyle of Alternaria brassicae - a pathogen of the Brassicaceae family.BMC genomics · 2019Article
- Deep Transcriptome Analysis Reveals Reactive Oxygen Species (ROS) Network Evolution, Response to Abiotic Stress, and Regulation of Fiber Development in Cotton.International journal of molecular sciences · 2019Article
- Article
- ScreeningMolecules (Basel, Switzerland) · 2018Article
- Article
- Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection.Frontiers in plant science · 2015Article
- ATP-sulfurylase, sulfur-compounds, and plant stress tolerance.Frontiers in plant science · 2015Review
- Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach.Journal of experimental botany · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In order to study the mechanisms behind the infection process of the necrotrophic fungus Botrytis cinerea, the subcellular distribution of hydrogen peroxide (H₂O₂) was monitored over a time frame of 96 h post inoculation (hpi) in Arabidopsis thaliana Col-0 leaves at the inoculation site (IS) and the area around the IS which was defined as area adjacent to the inoculation site (AIS). H₂O₂ accumulation was correlated with changes in the compartment-specific distribution of ascorbate and glutathione and chloroplast fine structure. This study revealed that the severe breakdown of the antioxidative system, indicated by a drop in ascorbate and glutathione contents at the IS at later stages of infection correlated with an accumulation of H₂O₂ in chloroplasts, mitochondria, cell walls, nuclei and the cytosol which resulted in the development of chlorosis and cell death, eventually visible as tissue necrosis. A steady increase of glutathione contents in most cell compartments within infected tissues (up to 600% in chloroplasts at 96 hpi) correlated with an accumulation of H₂O₂ in chloroplasts, mitochondria and cell walls at the AIS indicating that high glutathione levels could not prevent the accumulation of reactive oxygen species (ROS) which resulted in chlorosis. Summing up, this study reveals the intracellular sequence of events during Botrytis cinerea infection and shows that the breakdown of the antioxidative system correlated with the accumulation of H₂O₂ in the host cells. This resulted in the degeneration of the leaf indicated by severe changes in the number and ultrastructure of chloroplasts (e.g. decrease of chloroplast number, decrease of starch and thylakoid contents, increase of plastoglobuli size), chlorosis and necrosis of the leaves.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.