ArticlePloS one2014
Kidney specific protein-positive cells derived from embryonic stem cells reproduce tubular structures in vitro and differentiate into renal tubular cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Lineage tracing reveals KSPFrontiers in cell and developmental biology · 2026Article
- Assessing kidney development and disease using kidney organoids and CRISPR engineering.Frontiers in cell and developmental biology · 2022Review
- Human reconstructed kidney models.In vitro cellular & developmental biology. Animal · 2021Review
- Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers.NPJ Regenerative medicine · 2020Article
- Proximal tubule ATR regulates DNA repair to prevent maladaptive renal injury responses.The Journal of clinical investigation · 2019Article
- Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently.Scientific reports · 2019Article
- Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.Scientific reports · 2019Article
- Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis.Cancer management and research · 2019Article
- Concise Review: Kidney Generation with Human Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2017Review
- Hypoxia Inducible Factor 1: A Urinary Biomarker of Kidney Disease.Clinical and translational science · 2017Article
- Kidney Organoids: A Translational Journey.Trends in molecular medicine · 2017Review
- Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.Nature protocols · 2017Article
- Directing three-dimensional multicellular morphogenesis by self-organization of vascular mesenchymal cells in hyaluronic acid hydrogels.Journal of biological engineering · 2017Article
- Generation of kidney tubular organoids from human pluripotent stem cells.Scientific reports · 2016Article
- Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice.Cell reports · 2016Article
- Nephron organoids derived from human pluripotent stem cells model kidney development and injury.Nature biotechnology · 2015Article
- Meclizine Preconditioning Protects the Kidney Against Ischemia-Reperfusion Injury.EBioMedicine · 2015Article
- Article
- Directed Differentiation of Pluripotent Stem Cells into Kidney.Biomarker insights · 2015Review
- Three-dimensional organotypic culture: experimental models of mammalian biology and disease.Nature reviews. Molecular cell biology · 2014Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells and induced pluripotent stem cells have the ability to differentiate into various organs and tissues, and are regarded as new tools for the elucidation of disease mechanisms as well as sources for regenerative therapies. However, a method of inducing organ-specific cells from pluripotent stem cells is urgently needed. Although many scientists have been developing methods to induce various organ-specific cells from pluripotent stem cells, renal lineage cells have yet to be induced in vitro because of the complexity of kidney structures and the diversity of kidney-component cells. Here, we describe a method of inducing renal tubular cells from mouse embryonic stem cells via the cell purification of kidney specific protein (KSP)-positive cells using an anti-KSP antibody. The global gene expression profiles of KSP-positive cells derived from ES cells exhibited characteristics similar to those of cells in the developing kidney, and KSP-positive cells had the capacity to form tubular structures resembling renal tubular cells when grown in a 3D culture in Matrigel. Moreover, our results indicated that KSP-positive cells acquired the characteristics of each segment of renal tubular cells through tubular formation when stimulated with Wnt4. This method is an important step toward kidney disease research using pluripotent stem cells, and the development of kidney regeneration therapies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.