ArticlePloS one2014
Müller cell reactivity in response to photoreceptor degeneration in rats with defective polycystin-2.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 26 citations in OpenAlex.
- New Perspective on Aqueous Humor Circulation: Retina Takes the Lead.International journal of molecular sciences · 2025Review
- Regional Alterations in Müller Cell Protein Expression in Human and a Rat Model of Geographic Atrophy.Investigative ophthalmology & visual science · 2025Article
- MicroRNA-124 Alleviates Retinal Vasoregression via Regulating Microglial Polarization.International journal of molecular sciences · 2021Article
- Kir4.1 may represent a novel therapeutic target for diabetic retinopathy (Review).Experimental and therapeutic medicine · 2021Review
- Coordinated Intervention of Microglial and Müller Cells in Light-Induced Retinal Degeneration.Investigative ophthalmology & visual science · 2020Article
- Protective effect of Soluble Epoxide Hydrolase Inhibition in Retinal Vasculopathy associated with Polycystic Kidney Disease.Theranostics · 2020Article
- Dorsal-Ventral Differences in Retinal Structure in the Pigmented Royal College of Surgeons Model of Retinal Degeneration.Frontiers in cellular neuroscience · 2020Article
- A Review of Last Decade Developments on Epiretinal Membrane Pathogenesis.Medical hypothesis, discovery & innovation ophthalmology journal · 2020Review
- Comparative Effects of Coenzyme Q10 orAntioxidants (Basel, Switzerland) · 2018Article
- Oxygen-Induced Retinopathy from Recurrent Intermittent Hypoxia Is Not Dependent on Resolution with Room Air or Oxygen, in Neonatal Rats.International journal of molecular sciences · 2018Article
- Cone dystrophy and ectopic synaptogenesis in a Cacna1f loss of function model of congenital stationary night blindness (CSNB2A).Channels (Austin, Tex.) · 2018Article
- Astrocyte structural reactivity and plasticity in models of retinal detachment.Experimental eye research · 2016Review
- Impaired Purinergic Regulation of the Glial (Müller) Cell Volume in the Retina of Transgenic Rats Expressing Defective Polycystin-2.Neurochemical research · 2016Article
- Astrocytes and Müller Cell Alterations During Retinal Degeneration in a Transgenic Rat Model of Retinitis Pigmentosa.Frontiers in cellular neuroscience · 2015Article
- Aquaporin-1 expression in proliferative vitreoretinopathy and in epiretinal membranes.TheScientificWorldJournal · 2014Article
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Authors and funding
10 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundRetinal degeneration in transgenic rats that express a mutant cilia gene polycystin-2 (CMV-PKD2(1/703)HA) is characterized by initial photoreceptor degeneration and glial activation, followed by vasoregression and neuronal degeneration (Feng et al., 2009, PLoS One 4: e7328). It is unknown whether glial activation contributes to neurovascular degeneration after photoreceptor degeneration. We characterized the reactivity of Müller glial cells in retinas of rats that express defective polycystin-2.
methodsAge-matched Sprague-Dawley rats served as control. Retinal slices were immunostained for intermediate filaments, the potassium channel Kir4.1, and aquaporins 1 and 4. The potassium conductance of isolated Müller cells was recorded by whole-cell patch clamping. The osmotic swelling characteristics of Müller cells were determined by superfusion of retinal slices with a hypoosmotic solution.
findingsMüller cells in retinas of transgenic rats displayed upregulation of GFAP and nestin which was not observed in control cells. Whereas aquaporin-1 labeling of photoreceptor cells disappeared along with the degeneration of the cells, aquaporin-1 emerged in glial cells in the inner retina of transgenic rats. Aquaporin-4 was upregulated around degenerating photoreceptor cells. There was an age-dependent redistribution of Kir4.1 in retinas of transgenic rats, with a more even distribution along glial membranes and a downregulation of perivascular Kir4.1. Müller cells of transgenic rats displayed a slight decrease in their Kir conductance as compared to control. Müller cells in retinal tissues from transgenic rats swelled immediately under hypoosmotic stress; this was not observed in control cells. Osmotic swelling was induced by oxidative-nitrosative stress, mitochondrial dysfunction, and inflammatory lipid mediators.
interpretationCellular swelling suggests that the rapid water transport through Müller cells in response to osmotic stress is altered as compared to control. The dislocation of Kir4.1 will disturb the retinal potassium and water homeostasis, and osmotic generation of free radicals and inflammatory lipids may contribute to neurovascular injury.
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