ArticleProceedings of the National Academy of Sciences of the United States of America2013
Evidence for glycoprotein transport into complex plastids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- CurT dosage quantitatively affects thylakoid structure and PSII performance in Synechocystis sp. PCC 6803.The Plant journal : for cell and molecular biology · 2026Article
- CurT/CURT1 proteins are involved in cell and chloroplast division coordination of cyanobacteria and green algae.Nature communications · 2025Article
- Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early PSII assembly factor.The Plant cell · 2025Article
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Thylakoid attachment to the plasma membrane in Synechocystis sp. PCC 6803 requires the AncM protein.The Plant cell · 2022Article
- Article
- N-linked glycosylation enzymes in the diatom Thalassiosira oceanica exhibit a diel cycle in transcript abundance and favor for NXT-type sites.Scientific reports · 2021Article
- Global Profiling of N-Glycoproteins and N-Glycans in the DiatomFrontiers in plant science · 2021Article
- Metabolic Innovations Underpinning the Origin and Diversification of the Diatom Chloroplast.Biomolecules · 2019Review
- Dynamic N-glycoproteome analysis of maize seedling leaves during de-etiolation using Concanavalin A lectin affinity chromatography and a nano-LC-MS/MS-based iTRAQ approach.Plant cell reports · 2017Article
- Review: origin of complex algae by secondary endosymbiosis: a journey through time.Protoplasma · 2017Review
- Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes.eLife · 2016Article
- Microglomerular Synaptic Complexes in the Sky-Compass Network of the Honeybee Connect Parallel Pathways from the Anterior Optic Tubercle to the Central Complex.Frontiers in behavioral neuroscience · 2016Article
- New mode of energy metabolism in the seventh order of methanogens as revealed by comparative genome analysis of “Candidatus methanoplasma termitum”.Applied and environmental microbiology · 2015Article
- Oleosome-associated protein of the oleaginous diatom Fistulifera solaris contains an endoplasmic reticulum-targeting signal sequence.Marine drugs · 2014Article
- Glycoprotein import: a common feature of complex plastids?Plant signaling & behavior · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diatoms are microalgae that possess so-called "complex plastids," which evolved by secondary endosymbiosis and are surrounded by four membranes. Thus, in contrast to primary plastids, which are surrounded by only two membranes, nucleus-encoded proteins of complex plastids face additional barriers, i.e., during evolution, mechanisms had to evolve to transport preproteins across all four membranes. This study reveals that there exist glycoproteins not only in primary but also in complex plastids, making transport issues even more complicated, as most translocation machineries are not believed to be able to transport bulky proteins. We show that plastidal reporter proteins with artificial N-glycosylation sites are indeed glycosylated during transport into the complex plastid of the diatom Phaeodactylum tricornutum. Additionally, we identified five endogenous glycoproteins, which are transported into different compartments of the complex plastid. These proteins get N-glycosylated during transport across the outermost plastid membrane and thereafter are transported across the second, third, and fourth plastid membranes in the case of stromal proteins. The results of this study provide insights into the evolutionary pressure on translocation mechanisms and pose unique questions on the operating mode of well-known transport machineries like the translocons of the outer/inner chloroplast membranes (Toc/Tic).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.