ArticleProceedings of the National Academy of Sciences of the United States of America2013
Ptr-miR397a is a negative regulator of laccase genes affecting lignin content in Populus trichocarpa.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 217 papers.
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Who cites it
217 citing papers in PubMed, 396 citations in OpenAlex.
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- MicroRNA-Mediated Post-transcriptional Regulation of Wood Property Traits in Eucalyptus tereticornis.Biochemical genetics · 2026Article
- The Dual Role of Lignin in Fruit Trees: Unraveling Regulatory Networks from Stress Resilience to Quality Control.Plants (Basel, Switzerland) · 2026Review
- Genome-Wide Identification, Expression Profiling, and microRNA397-Mediated Regulation of Laccase Genes inPlants (Basel, Switzerland) · 2026Article
- Natural variation inScience advances · 2026Article
- Gallic Acid-Responsive microRNAs Reprogram Lignification During Drought Acclimation Process in Spearmint.Plant biotechnology journal · 2026Article
- Genome-wide identification, classification and expression analysis of laccase family members in Solanum lycopersicum.BMC plant biology · 2026Article
- An Integrative Small RNA-Degradome-Transcriptome Analysis Reveals Mechanisms of Heat-Induced Anther Indehiscence in Pepper.Biology · 2026Article
- Integrated metabolomics, PacBio SMRT, and Illumina sequencing analyses provide insights into molecular profiles associated with radial trunk growth in Chinese fir (Frontiers in plant science · 2026Article
- Characteristics and Molecular Mechanism of Slow Vegetative Growth in Hexaploid Transgenic Poplar 741 With an Insect-Resistance Gene.Plant biotechnology journal · 2025Article
- The Role of MicroRNA-Based Strategies in Optimizing Plant Biomass Composition for Bio-Based Packaging Materials.Plants (Basel, Switzerland) · 2025Review
- Factors underlying a latitudinal gradient in the S/G lignin monomer ratio in natural poplar variants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit.Journal of advanced research · 2025Article
- Validation of Fiber-Dominant Expressing Gene Promoters inPlants (Basel, Switzerland) · 2025Article
- Comprehensive analysis of cinnamoyl-CoA reductase (CCR) gene family in wheat: implications for lignin biosynthesis and stress responses.BMC plant biology · 2025Article
- Transposon-Associated Small RNAs Involved in Plant Defense in Poplar.Plants (Basel, Switzerland) · 2025Article
- Fatty Acid and Lipid Metabolism in Oil Palm: From Biochemistry to Molecular Mechanisms.International journal of molecular sciences · 2025Review
- Polymerization of proanthocyanidins under the catalysis of miR397a-regulated laccases in Salvia miltiorrhiza and Populus trichocarpa.Nature communications · 2025Article
- Silencing of miR169a improves drought stress by enhancing vascular architecture, ROS scavenging, and photosynthesis ofFrontiers in plant science · 2025Article
157 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laccases, as early as 1959, were proposed to catalyze the oxidative polymerization of monolignols. Genetic evidence in support of this hypothesis has been elusive due to functional redundancy of laccase genes. An Arabidopsis double mutant demonstrated the involvement of laccases in lignin biosynthesis. We previously identified a subset of laccase genes to be targets of a microRNA (miRNA) ptr-miR397a in Populus trichocarpa. To elucidate the roles of ptr-miR397a and its targets, we characterized the laccase gene family and identified 49 laccase gene models, of which 29 were predicted to be targets of ptr-miR397a. We overexpressed Ptr-MIR397a in transgenic P. trichocarpa. In each of all nine transgenic lines tested, 17 PtrLACs were down-regulated as analyzed by RNA-seq. Transgenic lines with severe reduction in the expression of these laccase genes resulted in an ∼40% decrease in the total laccase activity. Overexpression of Ptr-MIR397a in these transgenic lines also reduced lignin content, whereas levels of all monolignol biosynthetic gene transcripts remained unchanged. A hierarchical genetic regulatory network (GRN) built by a bottom-up graphic Gaussian model algorithm provides additional support for a role of ptr-miR397a as a negative regulator of laccases for lignin biosynthesis. Full transcriptome-based differential gene expression in the overexpressed transgenics and protein domain analyses implicate previously unidentified transcription factors and their targets in an extended hierarchical GRN including ptr-miR397a and laccases that coregulate lignin biosynthesis in wood formation. Ptr-miR397a, laccases, and other regulatory components of this network may provide additional strategies for genetic manipulation of lignin content.
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