ArticleLab on a chip2013
Superimposed topographic and chemical cues synergistically guide neurite outgrowth.
Article in Lab on a chip, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Feedback-Driven Dynamical Model for Axonal Extension on Parallel Micropatterns.Biomimetics (Basel, Switzerland) · 2025Article
- Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications.Small science · 2025Review
- Mechanical confinement matters: Unveiling the effect of two-photon polymerized 2.5D and 3D microarchitectures on neuronal YAP expression and neurite outgrowth.Materials today. Bio · 2024Article
- Recent advances in enhances peripheral nerve orientation: the synergy of micro or nano patterns with therapeutic tactics.Journal of nanobiotechnology · 2024Review
- Nonlinear Growth Dynamics of Neuronal Cells Cultured on Directional Surfaces.Biomimetics (Basel, Switzerland) · 2024Article
- Biased Random Walk Model of Neuronal Dynamics on Substrates with Periodic Geometrical Patterns.Biomimetics (Basel, Switzerland) · 2023Article
- Microfluidic Manipulation for Biomedical Applications in the Central and Peripheral Nervous Systems.Pharmaceutics · 2023Review
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- Feedback-controlled dynamics of neuronal cells on directional surfaces.Biophysical journal · 2022Article
- Modelling the central nervous system: tissue engineering of the cellular microenvironment.Emerging topics in life sciences · 2021Review
- Anisometric Microstructures to Determine Minimal Critical Physical Cues Required for Neurite Alignment.Advanced healthcare materials · 2021Article
- Neuronal Growth and Formation of Neuron Networks on Directional Surfaces.Biomimetics (Basel, Switzerland) · 2021Article
- Article
- Bioprinting Neural Systems to Model Central Nervous System Diseases.Advanced functional materials · 2020Review
- Cellular and Subcellular Contact Guidance on Microfabricated Substrates.Frontiers in bioengineering and biotechnology · 2020Review
- Article
- Advantageous environment of micro-patterned, high-density complementary metal-oxide-semiconductor electrode array for spiral ganglion neurons cultured in vitro.Scientific reports · 2018Article
- Biofunctionalized 3D Nanopillar Arrays Fostering Cell Guidance and Promoting Synapse Stability and Neuronal Activity in Networks.ACS applied materials & interfaces · 2018Article
- Tuning Surface and Topographical Features to Investigate Competitive Guidance of Spiral Ganglion Neurons.ACS applied materials & interfaces · 2017Article
- Development of a shear stress-free microfluidic gradient generator capable of quantitatively analyzing single-cell morphology.Biomedical microdevices · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Guidance of neuronal extensions is a complex process essential for linking neurons into complex functional networks underlying the workings of the neural system. Decades of research have suggested the ability of neuronal growth cones to integrate multiple types of cues during the extension process, but also have raised numerous still unanswered questions about synergy or antagonism between the superimposed chemical and mechanical signaling inputs. In this study, using a novel microfabricated analysis platform, we investigate the response of primary mouse embryonic hippocampal neurons to superimposed topographic and soluble chemical cues. We find that an optimal spatial frequency of topographic cues exists, maximizing the precision of the neurite extension. This optimal frequency can help the extending neurites navigate a topographically complex environment, providing pronounced directional selectivity. We also demonstrate that this cue can synergistically enhance attractive and suppress repulsive guidance by the bi-functional soluble cue Netrin-1, and eliminate the repulsive guidance by a chemorepellent Semaphorin3A (Sema3A). These results suggest that topographic cues can provide optimal periodic input into the guidance signaling processes involved in growth cone chemoattraction and can synergistically interact with chemical gradients of soluble guidance cues, shedding light on complex events accompanying the development of the functional nervous system.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.