ArticleAntimicrobial agents and chemotherapy2013
Distinction between the Cfr methyltransferase conferring antibiotic resistance and the housekeeping RlmN methyltransferase.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- A genomic framework for tracking antibiotic resistance genes: global dissemination ofAntimicrobial agents and chemotherapy · 2026Article
- Direct RNA Sequencing Reveals Stress-Dependent and Pathway-Specific rRNA Modification Reprogramming during 50S Biogenesis.Biochemistry · 2026Article
- Structural Analysis of 23S rRNA Methylating Enzyme Cfr Reveals RNA-Binding Determinants for Methylation Regiospecificity and Antibiotic Resistance.ACS infectious diseases · 2026Article
- Direct RNA Sequencing Reveals Stress-Dependent and Pathway-Specific rRNA Modification Reprogramming During 50S Biogenesis.bioRxiv : the preprint server for biology · 2026Article
- Genomic islands and molecular mechanisms relating to drug-resistance inEmerging microbes & infections · 2025Article
- Structural analysis of 23S rRNA methylating enzyme Cfr reveals RNA binding determinants for methylation regiospecificity and antibiotic resistance.bioRxiv : the preprint server for biology · 2025Article
- Linezolid-Resistant Coagulase-Negative Staphylococci in a Tertiary Hospital: Molecular Epidemiology, Clinical Characteristics, and Outcomes.Infection & chemotherapy · 2025Article
- Unravelling Antimicrobial Resistance inVeterinary sciences · 2024Review
- Structural basis of Cfr-mediated antimicrobial resistance and mechanisms to evade it.Nature chemical biology · 2024Article
- Phylogeny of Transferable Oxazolidinone Resistance Genes and Homologs.Antibiotics (Basel, Switzerland) · 2024Article
- CNature communications · 2024Article
- A Comprehensive Self-Resistance Gene Database for Natural-Product Discovery with an Application to Marine Bacterial Genome Mining.International journal of molecular sciences · 2023Article
- An RNA modification enzyme directly senses reactive oxygen species for translational regulation in Enterococcus faecalis.Nature communications · 2023Article
- Circular intermediate-mediated horizontal transfer of the chromosome-encodedFrontiers in microbiology · 2023Article
- ARTS-DB: a database for antibiotic resistant targets.Nucleic acids research · 2022Article
- Article
- Target protection as a key antibiotic resistance mechanism.Nature reviews. Microbiology · 2020Review
- Analysis of RNA Methylation by Phylogenetically Diverse Cfr RadicalBiochemistry · 2019Article
- miCLIP-MaPseq, a Substrate Identification Approach for Radical SAM RNA Methylating Enzymes.Journal of the American Chemical Society · 2018Article
- Elp3 and RlmN: A tale of two mitochondrial tail-anchored radical SAM enzymes in Toxoplasma gondii.PloS one · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cfr gene encodes the Cfr methyltransferase that primarily methylates C-8 in A2503 of 23S rRNA in the peptidyl transferase region of bacterial ribosomes. The methylation provides resistance to six classes of antibiotics of clinical and veterinary importance. The rlmN gene encodes the RlmN methyltransferase that methylates C-2 in A2503 in 23S rRNA and A37 in tRNA, but RlmN does not significantly influence antibiotic resistance. The enzymes are homologous and use the same mechanism involving radical S-adenosyl methionine to methylate RNA via an intermediate involving a methylated cysteine in the enzyme and a transient cross-linking to the RNA, but they differ in which carbon atom in the adenine they methylate. Comparative sequence analysis identifies differentially conserved residues that indicate functional sequence divergence between the two classes of Cfr- and RlmN-like sequences. The differentiation between the two classes is supported by previous and new experimental evidence from antibiotic resistance, primer extensions, and mass spectrometry. Finally, evolutionary aspects of the distribution of Cfr- and RlmN-like enzymes are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.