ArticleThe Journal of cell biology2013
A Wee1 checkpoint inhibits anaphase onset.
Article in The Journal of cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 66 citations in OpenAlex.
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- A cyclin-polarity feedback network ensures healthy cell proliferation.Nature communications · 2026Article
- Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin.bioRxiv : the preprint server for biology · 2026Article
- Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing.Nature communications · 2025Article
- CDC6 as a Key Inhibitory Regulator of CDK1 Activation Dynamics and the Timing of Mitotic Entry and Progression.Biology · 2023Review
- Evolving DNA repair synthetic lethality targets in cancer.Bioscience reports · 2022Article
- Article
- Protein phosphatase 2A (PP2A) promotes anaphase entry after DNA replication stress in budding yeast.Molecular biology of the cell · 2021Article
- Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension.The EMBO journal · 2021Article
- Kar9 symmetry breaking alone is insufficient to ensure spindle alignment.Scientific reports · 2021Article
- The mitotic checkpoint is a targetable vulnerability of carboplatin-resistant triple negative breast cancers.Scientific reports · 2021Article
- PP2AJournal of cell science · 2020Article
- Growth-Dependent Activation of Protein Kinases Suggests a Mechanism for Measuring Cell Growth.Genetics · 2020Article
- Yeast Sphingolipid Phospholipase GeneMolecular and cellular biology · 2020Article
- Haspin Modulates the G2/M Transition Delay in Response to Polarization Failures in Budding Yeast.Frontiers in cell and developmental biology · 2020Article
- PP2A Functions during Mitosis and Cytokinesis in Yeasts.International journal of molecular sciences · 2019Review
- A Conserved PP2A Regulatory Subunit Enforces Proportional Relationships Between Cell Size and Growth Rate.Genetics · 2019Article
- Prolonged cyclin-dependent kinase inhibition results in septin perturbations during return to growth and mitosis.The Journal of cell biology · 2018Article
- SILAC-based phosphoproteomics reveals new PP2A-Cdc55-regulated processes in budding yeast.GigaScience · 2018Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Cdk1 drives both mitotic entry and the metaphase-to-anaphase transition. Past work has shown that Wee1 inhibition of Cdk1 blocks mitotic entry. Here we show that the budding yeast Wee1 kinase, Swe1, also restrains the metaphase-to-anaphase transition by preventing Cdk1 phosphorylation and activation of the mitotic form of the anaphase-promoting complex/cyclosome (APC(Cdc20)). Deletion of SWE1 or its opposing phosphatase MIH1 (the budding yeast cdc25(+)) altered the timing of anaphase onset, and activation of the Swe1-dependent morphogenesis checkpoint or overexpression of Swe1 blocked cells in metaphase with reduced APC activity in vivo and in vitro. The morphogenesis checkpoint also depended on Cdc55, a regulatory subunit of protein phosphatase 2A (PP2A). cdc55Δ checkpoint defects were rescued by mutating 12 Cdk1 phosphorylation sites on the APC, demonstrating that the APC is a target of this checkpoint. These data suggest a model in which stepwise activation of Cdk1 and inhibition of PP2A(Cdc55) triggers anaphase onset.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.