Evidence map›Paper›PMID 23751346›Full record

ArticleBiochemical and biophysical research communications2013

Ambient ultrafine particles reduce endothelial nitric oxide production via S-glutathionylation of eNOS.

Yunfeng Du, Mohamad Navab, Melody Shen, James Hill, Payam Pakbin, Constantinos Sioutas, Tzung K Hsiai, Rongsong Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yunfeng DuBiomedical Engineering, University of Southern California, Los Angeles, CA 90089, United States.
Mohamad Navab
Melody Shen
James Hill
Payam Pakbin
Constantinos Sioutas
Tzung K Hsiai
Rongsong Li
University of Southern California · USChangzhou No.2 People's Hospital · CNUniversity of California, Los Angeles · US

Funding

Linking Spatial Variations Shear Stress-Oxidative StressR01HL083015 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HSIAI, TZUNG K · 2006 to 2015
$3.9M
Electronica Impedance to Access Metabolically Active PlaqueR01HL118650 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K · 2014 to 2022
$3.3M
Micro-Sensors to Study Electrical and Mechanical Coupling of Injured MyocardiumR01HL111437 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HSIAI, TZUNG K, XU, XIAOLEI · 2012 to 2020
$3.1M
NHLBI NIH HHS R01 HL083015NHLBI NIH HHS R01HL083015NHLBI NIH HHS R01 HL111437NHLBI NIH HHS R01HL111437NHLBI NIH HHS R01 HL118650
6 · The paper itself

Abstract

Exposure to airborne particulate pollutants is intimately linked to vascular oxidative stress and inflammatory responses with clinical relevance to atherosclerosis. Particulate matter (PM) has been reported to induce endothelial dysfunction and atherosclerosis. Here, we tested whether ambient ultrafine particles (UFP, diameter <200 nm) modulate eNOS activity in terms of nitric oxide (NO) production via protein S-glutathionylation. Treatment of human aortic endothelial cells (HAEC) with UFP significantly reduced NO production. UFP-mediated reduction in NO production was restored in the presence of JNK inhibitor (SP600125), NADPH oxidase inhibitor (Apocynin), anti-oxidant (N-acetyl cysteine), and superoxide dismutase mimetics (Tempol and MnTMPyP). UFP exposure increased the GSSG/GSH ratio and eNOS S-glutathionylation, whereas over-expression of Glutaredoxin-1 (to inhibit S-glutathionylation) restored UFP-mediated reduction in NO production by nearly 80%. Thus, our findings suggest that eNOS S-glutathionylation is a potential mechanism underlying ambient UFP-induced reduction of NO production.

Indexed as

Particle SizeAcetophenonesAnimalsAnthracenesAortaBiomimeticsCells, CulturedCyclic N-OxidesEndothelial CellsEndothelium, VascularEnzyme ActivationGlutathioneHumansMetalloporphyrinsMiceNADPH OxidasesAcetophenonesacetovanilloneAnthracenesCyclic N-OxidesGlutathioneMetalloporphyrinsMn(III) 5,10,15,20-tetrakis(N-methylpyridinium-2-yl)porphyrinNADPH OxidasesNitric OxideNitric Oxide Synthase Type IIINOS3 protein, humanParticulate MatterpyrazolanthroneSOD1 protein, humanSod1 protein, mouseSpin LabelsSuperoxide DismutaseSuperoxide Dismutase-1tempolAir pollutionEndothelial dysfunctioneNOSOxidative stressS-glutathionylationUltrafine particles/UFP

Identifiers

PMID23751346
PMCPMC3743434
OpenAlexW2081458682

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.