ArticleCellular microbiology2013
Open membranes are the precursors for assembly of large DNA viruses.
Article in Cellular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Infection cycles of viruses of the phylum Nucleocytoviricota.Nature reviews. Microbiology · 2026Review
- Elucidating the protein interaction network of one of the largest icosahedral capsids in the virosphere.The EMBO journal · 2026Article
- Article
- Scanning transmission electron tomography to study virus assembly: Review for the retirement of Paul Walther.Journal of microscopy · 2025Review
- Acanthamoeba polyphaga de novo transcriptome and its dynamics during Mimivirus infection.Scientific reports · 2024Article
- Assembly and Evolution of Poxviruses.Advances in experimental medicine and biology · 2024Review
- Architecture and Assembly of Structurally Complex Viruses.Sub-cellular biochemistry · 2024Review
- Virologs, viral mimicry, and virocell metabolism: the expanding scale of cellular functions encoded in the complex genomes of giant viruses.FEMS microbiology reviews · 2023Article
- Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly.Nature communications · 2023Article
- Particle Morphology of Medusavirus Inside and Outside the Cells Reveals a New Maturation Process of Giant Viruses.Journal of virology · 2022Article
- Article
- Current capsid assembly models of icosahedral nucleocytoviricota viruses.Advances in virus research · 2020Review
- Chloroviruses.Viruses · 2019Review
- RNA interference identifies domesticated viral genes involved in assembly and trafficking of virus-derived particles in ichneumonid wasps.PLoS pathogens · 2019Article
- Complex Membrane Remodeling during Virion Assembly of the 30,000-Year-Old Mollivirus Sibericum.Journal of virology · 2019Article
- The Roles of Electrostatic Interactions in Capsid Assembly Mechanisms of Giant Viruses.International journal of molecular sciences · 2019Article
- New Isolates of Pandoraviruses: Contribution to the Study of Replication Cycle Steps.Journal of virology · 2019Article
- The viral replication organelles within cells studied by electron microscopy.Advances in virus research · 2019Article
- Origin of the poxviral membrane: A 50-year-old riddle.PLoS pathogens · 2018Review
- Cedratvirus getuliensis replication cycle: an in-depth morphological analysis.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Nucleo cytoplasmic large DNA viruses (NCLDVs) are a group of double-stranded DNA viruses that replicate their DNA partly or entirely in the cytoplasm in association with viral factories (VFs). They share about 50 genes suggesting that they are derived from a common ancestor. Using transmission electron microscopy (TEM) and electron tomography (ET) we showed that the NCLDV vaccinia virus (VACV) acquires its membrane from open membrane intermediates, derived from the ER. These open membranes contribute to the formation of a single open membrane of the immature virion, shaped into a sphere by the assembly of the viral scaffold protein on its convex side. We now compare VACV with the NCLDV Mimivirus by TEM and ET and show that the latter also acquires its membrane from open membrane intermediates that accumulate at the periphery of the cytoplasmic VF. In analogy to VACV this membrane is shaped by the assembly of a layer on the convexside of its membrane, likely representing the Mimivirus capsid protein. By quantitative ET we show for both viruses that the open membrane intermediates of assembly adopt an 'open-eight' conformation with a characteristic diameter of 90 nm for Mimi- and 50 nm for VACV. We discuss these results with respect to the common ancestry of NCLDVs and propose a hypothesis on the possible origin of this unusual membrane biogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.