Evidence map›Paper›PMID 23750543›Full record

ArticleBioTechniques2013

Production of single-stranded DNAs by self-cleavage of rolling-circle amplification products.

Hongzhou Gu, Ronald R Breaker

Open access · goldAbstract read
In one paragraph

Article in BioTechniques, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 31 citations in OpenAlex.

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  12. Small, highly active DNAs that hydrolyze DNA.Journal of the American Chemical Society · 2013
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Hongzhou GuDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.
Ronald R Breaker
Howard Hughes Medical Institute · US

Funding

X-RAY ANALYSIS OF PROTEIN STRUCTURE AND FUNCTIONP01GM022778 · NIGMS · YALE UNIVERSITY · PI BREAKER, RONALD R · 1985 to 2019
$26.1M
Howard Hughes Medical InstituteNIGMS NIH HHS GM022778NIGMS NIH HHS P01 GM022778
6 · The paper itself

Abstract

DNA molecules that encode a small, high-speed self-hydrolyzing deoxyribozyme are used as templates for rolling circle amplification (RCA) to produce single-stranded DNAs (ssDNAs) of single- and multiple-unit lengths. Including self-cleaving deoxyribozymes in RCA products can generate large amounts of ssDNAs with defined sequence and length as well as precise termini. We also demonstrate the use of this method to efficiently generate ssDNA size markers by using deoxyribozyme reaction conditions that permit partial processing.

Indexed as

DNA, CatalyticDNA, Single-StrandedElectrophoresis, Agar GelNucleic Acid Amplification TechniquesNucleic Acid ConformationRNADNA, CatalyticDNA, Single-StrandedRNA

Identifiers

PMID23750543
PMCPMC5325123
OpenAlexW90085565

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.