ArticleJournal of bacteriology2013
Numbers of individual nutrient germinant receptors and other germination proteins in spores of Bacillus subtilis.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- A mathematical kinetic model of memory iniScience · 2026Article
- A Family of Spore Lipoproteins Stabilizes the Germination Apparatus by Altering Inner Spore Membrane Fluidity inJournal of bacteriology · 2023Article
- Changes in the Spore Proteome ofMicroorganisms · 2022Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne PathogenInternational journal of molecular sciences · 2021Article
- Membrane Proteomes and Ion Transporters inJournal of bacteriology · 2019Article
- Identification of L-Valine-initiated-germination-active genes in Bacillus subtilis using Tn-seq.PloS one · 2019Article
- Positions 299 and 302 of the GerAA subunit are important for function of the GerA spore germination receptor in Bacillus subtilis.PloS one · 2018Article
- Sporulation: how to survive on planet Earth (and beyond).Current genetics · 2017Review
- The Bacillus subtilis germinant receptor GerA triggers premature germination in response to morphological defects during sporulation.Molecular microbiology · 2017Article
- Bacillus thermoamylovorans Spores with Very-High-Level Heat Resistance Germinate Poorly in Rich Medium despite the Presence of ger Clusters but Efficiently upon Exposure to Calcium-Dipicolinic Acid.Applied and environmental microbiology · 2015Article
- Phenotypic Diversity as a Mechanism to Exit Cellular Dormancy.Current biology : CB · 2015Article
- The effects of heat activation on Bacillus spore germination, with nutrients or under high pressure, with or without various germination proteins.Applied and environmental microbiology · 2015Article
- Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.Journal of bacteriology · 2015Article
- Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores.Journal of bacteriology · 2014Article
- Structural and functional analysis of the GerD spore germination protein of Bacillus species.Journal of molecular biology · 2014Article
- L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.BMC microbiology · 2014Article
- Levels of germination proteins in Bacillus subtilis dormant, superdormant, and germinating spores.PloS one · 2014Article
- The Clostridium perfringens germinant receptor protein GerKC is located in the spore inner membrane and is crucial for spore germination.Journal of bacteriology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germination of dormant Bacillus subtilis spores with specific nutrient germinants is dependent on a number of inner membrane (IM) proteins, including (i) the GerA, GerB, and GerK germinant receptors (GRs) that respond to nutrient germinants; (ii) the GerD protein, essential for optimal GR function; and (iii) SpoVA proteins, essential for the release of the spore-specific molecule dipicolinic acid (DPA) during spore germination. Levels of GR A and C subunit proteins, GerD, and SpoVAD in wild-type spores were determined by Western blot analysis of spore fractions or total disrupted spores by comparison with known amounts of purified proteins. Surprisingly, after disruption of decoated B. subtilis spores with lysozyme and fractionation, ∼90% of IM fatty acids and GR subunits remained with the spores' insoluble integument fraction, indicating that yields of purified IM are low. The total lysate from disrupted wild-type spores contained ∼2,500 total GRs/spore: GerAA and GerAC subunits each at ∼1,100 molecules/spore and GerBC and GerKA subunits each at ∼700 molecules/spore. Levels of the GerBA subunit determined previously were also predicted to be ∼700 molecules/spore. These results indicate that the A/C subunit stoichiometry in GRs is most likely 1:1, with GerA being the most abundant GR. GerD and SpoVAD levels were ∼3,500 and ∼6,500 molecules/spore, respectively. These values will be helpful in formulating mathematic models of spore germination kinetics as well as setting lower limits on the size of the GR-GerD complex in the spores' IM, termed the germinosome.
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