ArticleHuman cell2013
Effects of extracellular calcium on viability and osteogenic differentiation of bone marrow stromal cells in vitro.
Article in Human cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Rapid mineralization of hydrothermally induced TiOJournal of materials science. Materials in medicine · 2026Article
- Osteogenic Effects of Ion Released from Biodegradable Metallic Magnesium and Calcium Coating.Advanced biology · 2025Article
- Responses of stem cells isolated from human primary teeth to calcium-containing pulp capping materials.BMC oral health · 2025Article
- PVP as an Oxygen Vacancy-Inducing Agent in the Development of Black 45S5 Bioactive Glass Fibrous Scaffolds Doped with Zn and Mg Using A-HSBS.Materials (Basel, Switzerland) · 2025Article
- Self-oxygenation of engineered living tissues orchestrates osteogenic commitment of mesenchymal stem cells.Biomaterials · 2023Article
- Decoupling the role of chemistry and microstructure in hMSCs response to an osteoinductive calcium phosphate ceramic.Bioactive materials · 2023Article
- Citrate Supplementation Restores the Impaired Mineralisation Resulting from the Acidic Microenvironment: An In Vitro Study.Nutrients · 2020Article
- iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy.Stem cell research & therapy · 2019Article
- Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression.Experimental & molecular medicine · 2018Article
- Article
- Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration.International journal of nanomedicine · 2017Article
- Clinical and Molecular Perspectives of Reparative Dentin Formation: Lessons Learned from Pulp-Capping Materials and the Emerging Roles of Calcium.Dental clinics of North America · 2017Review
- Promotion of in vivo degradability, vascularization and osteogenesis of calcium sulfate-based bone cements containing nanoporous lithium doping magnesium silicate.International journal of nanomedicine · 2017Article
- The Role of ORAI1 in the Odontogenic Differentiation of Human Dental Pulp Stem Cells.Journal of dental research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone marrow stromal cells (BMSCs) have been extensively used for tissue engineering. However, the effect of Ca(2+) on the viability and osteogenic differentiation of BMSCs has yet to be evaluated. To determine the dose-dependent effect of Ca(2+) on viability and osteogenesis of BMSCs in vitro, BMSCs were cultured in calcium-free DMEM medium supplemented with various concentrations of Ca(2+) (0, 1, 2, 3, 4, and 5 mM) from calcium citrate. Cell viability was analyzed by MTT assay and osteogenic differentiation was evaluated by alkaline phosphatase (ALP) assay, Von Kossa staining, and real-time PCR. Ca(2+) stimulated BMSCs viability in a dose-dependent manner. At slightly higher concentrations (4 and 5 mM) in the culture, Ca(2+) significantly inhibited the activity of ALP on days 7 and 14 (P < 0.01 or P < 0.05), significantly suppressed collagen synthesis (P < 0.01 or P < 0.05), and significantly elevated calcium deposition (P < 0.01) and mRNA levels of osteocalcin (P < 0.01 or P < 0.05) and osteopontin (P < 0.01 or P < 0.05). Therefore, elevated concentrations of extracellular calcium may promote cell viability and late-stage osteogenic differentiation, but may suppress early-stage osteogenic differentiation in BMSCs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.