ArticleInternational journal of legal medicine2013
FVIIIra, CD15, and tryptase performance in the diagnosis of skin stab wound vitality in forensic pathology.
Article in International journal of legal medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.
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Who cites it
14 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- State-of-the-Art on Wound Vitality Evaluation: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review.Frontiers in medicine · 2021Pooled it
- Histological and Immunohistochemical Biomarkers for Wound Age Estimation in Human Skin: A Systematic Review.Cureus · 2026Review
- Immunohistochemical analysis of PDGFR-α for wound age determination.International journal of legal medicine · 2024Article
- Skin injuries in forensic histopathology: a descriptive study.Forensic science, medicine, and pathology · 2024Article
- Dating Skin Lesions of Forensic Interest by Immunohistochemistry and Immunofluorescence Techniques: A Scoping Literature Review.Diagnostics (Basel, Switzerland) · 2024Article
- Novel ratio-expressions of genes enables estimation of wound age in contused skeletal muscle.International journal of legal medicine · 2024Article
- Myeloperoxydase and CD15 With Glycophorin C Double Staining in the Evaluation of Skin Wound Vitality in Forensic Practice.Frontiers in medicine · 2022Article
- Cytokines as new biomarkers of skin wound vitality.International journal of legal medicine · 2021Article
- Wound Vitality in Decomposed Bodies: New Frontiers Through Immunohistochemistry.Frontiers in medicine · 2021Article
- Vitality and wound-age estimation in forensic pathology: review and future prospects.Forensic sciences research · 2020Review
- CXCL1 and CXCR2 as potential markers for vital reactions in skin contusions.Forensic science, medicine, and pathology · 2018Article
- Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination.International journal of legal medicine · 2015Article
- Autophagy in skin wounds: a novel marker for vital reactions.International journal of legal medicine · 2015Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The timing of skin wounds is one of the most challenging problems in forensic pathology. In the first minutes or hours after infliction, histological examination fails to determine whether a wound was sustained before or after death. The aim of this study was to evaluate the use of three immunohistochemical markers (FVIIIra, CD15, and tryptase) for the interpretation of the timing of cutaneous stab wounds. We evaluated these markers in intravital wounds from autopsy cases (n = 12) and surgical specimens (n = 58). As controls, we used normal skin samples from autopsies (n = 8) and an original ex vivo surgical human model of recent postmortem wounds (n = 24). We found overexpression of FVIIIra in 100 % of vital wounds, but also in 53 % of the controls. The number of CD15-positive cells was higher in wound margins than in internal controls (p < 0.0001) and was significantly correlated with the time interval between incision and devascularization (p = 0.0005; minimal time for positivity, 9 min). Using the anti-tryptase antibody, we found that the mast cell degranulation rate was higher in wound margins (p < 0.0001) and correlated with the time interval (minimal time, 1 min). The sensitivity and specificity for the diagnosis of vitality were respectively 100 and 47 % for FVIIIra, 47 and 100 % for CD15, and 60 and 100 % for tryptase. The inter-observer agreement coefficients were 0.68 for FVIIIra, 0.90 for CD15, and 0.46 for tryptase. Finally, we demonstrated that these markers were not reliable in putrefied or desiccated specimens. In conclusion, CD15 and tryptase, but not FVIIIra, may be useful markers for differentiating recent antemortem from postmortem injuries.
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