ArticleInternational journal of legal medicine2014
The potential use of bacterial community succession in forensics as described by high throughput metagenomic sequencing.
Article in International journal of legal medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
110 citing papers in PubMed, 310 citations in OpenAlex.
- Temporal succession of microbiomes and resistomes during buried rat carcass decomposition.mSphere · 2026Article
- Microbiome modelling for post-mortem interval estimation across species and climates: swine analogues in a British summer and external validation in human donors from the USA.Scientific reports · 2026Article
- Microbial bioindicators for multidimensional assessment of environmental alterations and ecosystem functioning.World journal of microbiology & biotechnology · 2026Review
- A VOC-centric web of biological interaction on corpse: Consideration for forensic PMI estimation.iScience · 2026Review
- Differentiating hemorrhagic shock and organophosphate poisoning through integrated skin microbiome-metabolome signatures.BMC microbiology · 2026Article
- Insects shape the cadaver decomposition microbiome and postmortem interval estimation accuracy.mSystems · 2026Article
- Microbial succession and machine learning for postmortem interval estimation in bromadiolone-poisoned cadavers.AMB Express · 2026Article
- Postmortem Microbiology in Forensic Diagnostics: Interpretation of Infectious Causes of Death and Emerging Applications.Diagnostics (Basel, Switzerland) · 2026Review
- Microbial succession in human tissues postmortem: insights from 2bRAD-M sequencing.Microbiology spectrum · 2026Article
- The impact of age and insects factors on cadaver microbial communities and application to postmortem interval Estimation.International journal of legal medicine · 2026Article
- Temporal Dynamics of Bacterial Communities inInsects · 2025Article
- The microbiome and volatile organic compounds reflecting the state of decomposition in an indoor environment.Science & justice : journal of the Forensic Science Society · 2025Article
- Current Understanding and Future Research Direction for Estimating the Postmortem Interval: A Systematic Review.Diagnostics (Basel, Switzerland) · 2025Review
- Unveiling the Forensic Potential of Oral and Nasal Microbiota in Post-Mortem Interval Estimation.International journal of molecular sciences · 2025Article
- Potential postmortem microbial biomarkers of infant and younger children death investigation.Journal of forensic sciences · 2025Article
- Advancing time-since-interval estimation for clandestine graves: a forensic ecogenomics perspective into burial and translocation timelines using massively parallel sequencing.Frontiers in microbiology · 2025Review
- Exploring vaginal microbiome: from traditional methods to metagenomic next-generation sequencing-a systematic review.Frontiers in microbiology · 2025Review
- RedefiningFrontiers in microbiology · 2025Review
- The Functional and Structural Succession of Mesic-Grassland Soil Microbiomes Beneath Decomposing Large Herbivore Carcasses.Environmental microbiology · 2025Article
- Influence of Drugs and Toxins on Decomposition Dynamics: Forensic Implications.Molecules (Basel, Switzerland) · 2024Review
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Decomposition studies of vertebrate remains primarily focus on data that can be seen with the naked eye, such as arthropod or vertebrate scavenger activity, with little regard for what might be occurring with the microorganism community. Here, we discuss the necrobiome, or community of organisms associated with the decomposition of remains, specifically, the "epinecrotic" bacterial community succession throughout decomposition of vertebrate carrion. Pyrosequencing was used to (1) detect and identify bacterial community abundance patterns that described discrete time points of the decomposition process and (2) identify bacterial taxa important for estimating physiological time, a time-temperature metric that is often commensurate with minimum post-mortem interval estimates, via thermal summation models. There were significant bacterial community structure differences in taxon richness and relative abundance patterns through the decomposition process at both phylum and family taxonomic classification levels. We found a significant negative linear relationship for overall phylum and family taxon richness as decomposition progressed. Additionally, we developed a statistical model using high throughput sequencing data of epinecrotic bacterial communities on vertebrate remains that explained 94.4 % of the time since placement of remains in the field, which was within 2-3 h of death. These bacteria taxa are potentially useful for estimating the minimum post-mortem interval. Lastly, we provide a new framework and standard operating procedure of how this novel approach of using high throughput metagenomic sequencing has remarkable potential as a new forensic tool. Documenting and identifying differences in bacterial communities is key to advancing knowledge of the carrion necrobiome and its applicability in forensic science.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.