ArticleParasitology research2013
Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan.
Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- High prevalence ofParasite epidemiology and control · 2026Article
- Identification of Acantamoeba isolated from water resources located in the northwest of Iran by using the high-resolution melting analysis assay.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Identification and genotyping ofParasite epidemiology and control · 2023Article
- Distribution and Current State of Molecular Genetic Characterization in Pathogenic Free-Living Amoebae.Pathogens (Basel, Switzerland) · 2022Review
- Free-Living Amoebas in Extreme Environments: The True Survival in our Planet.BioMed research international · 2022Review
- On the diversity and clinical importance of Acanthamoeba spp. from Group 1.Parasitology research · 2021Article
- Isolation, identification of pathogenicJournal of advanced veterinary and animal research · 2018Article
- The cellulose synthase BcsA plays a role in interactions of Salmonella typhimurium with Acanthamoeba castellanii genotype T4.Parasitology research · 2018Article
- Article
- Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt.Parasitology research · 2016Article
- Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods.Parasitology research · 2016Article
- Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp. nov. (genotype T19).Parasitology research · 2015Article
- Molecular Characterization of Pathogenic Acanthamoeba Isolated from Drinking and Recreational water in East Azerbaijan, Northwest Iran.Environmental health insights · 2015Article
- The Rising Dominance of Shigella sonnei: An Intercontinental Shift in the Etiology of Bacillary Dysentery.PLoS neglected tropical diseases · 2015Review
- Isolation and molecular characterization of Acanthamoeba and Balamuthia mandrillaris from combination shower units in Costa Rica.Parasitology research · 2014Article
- Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain.Parasitology research · 2014Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba, an opportunistic protozoan pathogen, is ubiquitous in nature, and therefore plays a predatory role and helps control microbial communities in the ecosystem. These Acanthamoeba species are recognized as opportunistic human pathogens that may cause blinding keratitis and rare but fatal granulomatous encephalitis. To date, there is not a single report demonstrating Acanthamoeba isolation and identification from environmental sources in Pakistan, and that is the aim of this study. Acanthamoeba were identified by morphological characteristics of their cysts on non-nutrient agar plates seeded with Escherichia coli. Additionally, the polymerase chain reaction (PCR) was performed with genus-specific primers followed by direct sequencing of the PCR product for molecular identification. Furthermore, our PCR and sequencing results confirmed seven different pathogenic and nonpathogenic genotypes, including T2-T10, T4, T5, T7, T15, T16, and T17. To the best of our knowledge, we have identified and isolated Acanthamoeba sp., for the first time, from water resources of Khyber Pakhtunkhwa, Pakistan. There is an urgent need to address (1) the pathogenic potential of the identified genotypes and (2) explore other environmental sources from the country to examine the water quality and the current status of Acanthamoeba species in Pakistan, which may be a potential threat for public health across the country.
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