ArticleTransgenic research2013
A proteomic approach to study the mechanism of tolerance to Bt toxins in Ostrinia furnacalis larvae selected for resistance to Cry1Ab.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Comparative Analysis of Insect Resistance in TransgenicPlants (Basel, Switzerland) · 2025Article
- Insect chaperones Hsp70 and Hsp90 cooperatively enhance toxicity ofFrontiers in immunology · 2023Article
- Activation of Bacillus thuringiensis Cry1I to a 50 kDa stable core impairs its full toxicity to Ostrinia nubilalis.Applied microbiology and biotechnology · 2022Article
- Exogenous Gene Expression and Insect Resistance in Dual Bt ToxinFrontiers in plant science · 2021Article
- Synergistic Effect ofInternational journal of molecular sciences · 2020Article
- Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer.Toxins · 2020Article
- CRISPR-Mediated Knockout of theToxins · 2020Article
- Study of theToxins · 2020Article
- Article
- No impact of transgenic cry1Ie maize on the diversity, abundance and composition of soil fauna in a 2-year field trial.Scientific reports · 2019Article
- Identification and Gene Expression Analysis of the Pheromone Biosynthesis Activating Neuropeptide Receptor (PBANR) From the Ostrinia furnacalis (Lepidoptera: Pyralidae).Journal of insect science (Online) · 2019Article
- Transcriptome and Proteome Alternation With Resistance toFrontiers in physiology · 2019Article
- Transcriptomic Responses to Different Cry1Ac Selection Stresses inFrontiers in physiology · 2018Article
- Article
- Proteomic analysis of Cry2Aa-binding proteins and their receptor function in Spodoptera exigua.Scientific reports · 2017Article
- Effects of transgenic cry1Ie maize on non-lepidopteran pest abundance, diversity and community composition.Transgenic research · 2016Article
- Field trials to evaluate the effects of transgenic cry1Ie maize on the community characteristics of arthropod natural enemies.Scientific reports · 2016Article
- Identification of differentially expressed microRNAs between Bacillus thuringiensis Cry1Ab-resistant and -susceptible strains of Ostrinia furnacalis.Scientific reports · 2015Article
- Transcriptional analysis of susceptible and resistant European corn borer strains and their response to Cry1F protoxin.BMC genomics · 2015Article
- Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer.BMC genomics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Cry1Ab-resistant population of Asian corn borer (ACB-AbR) exhibiting approximately 100 times greater resistance to activated Cry1Ab than a susceptible population (Ostrinia furnacalis; ACB-BtS), was previously shown to exhibit high levels of cross-resistance to Cry1Ah (131-fold), but no cross-resistance to Cry1Ie. It was suggested that the proposed mechanism of resistance was due to the alteration of specific receptors for Cry toxins in the midgut brush border membrane. In the present study a proteomic-based approach was used to identify proteins from brush border membrane vesicles (isolated from both resistant and susceptible Ostrinia furnacalis larvae) interacting with biotinylated Cry1Ab, Cry1Ah, and Cry1Ie. 2D-Electrophoresis in combination with ligand blots were employed and putative protein identities obtained using MALDI-ToF/ToF mass spectrometry. The V-type proton ATPase catalytic subunit A and heat shock 70 kDa proteins were identified as interacting with the Cry toxins tested in the ACB-AbR and ACB-BtS larvae. The biotinylated Cry toxins showed markedly stronger interactions with proteins in the resistant compared to the susceptible larvae, suggesting an up-regulation of the V-type proton ATPase catalytic subunit A and heat shock 70 kDa proteins in the resistant (ACB-AbR) larvae. Interestingly, Cry1Ie interactions with the V-type proton ATPase catalytic subunit A in the ACB-BtS larvae appeared to be absent.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.