ArticleTransgenic research2013
Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte).
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 90 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 233 citations in OpenAlex.
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- MiSiPi.Rna: an integrated tool for characterizing small regulatory RNA processing.RNA (New York, N.Y.) · 2026Article
- Self-Assembling Nanoparticle Enhances RNA Interference Efficiency againstJournal of agricultural and food chemistry · 2026Article
- RNAi-mediated gene silencing of a 26S proteasome subunit increases mortality of the Japanese beetle Popillia japonica.Pest management science · 2026Article
- Next-generation insect pest management: genetic innovations and emerging biocontrol strategies.Frontiers in plant science · 2026Review
- Evaluating the new product Norroa™ againstFrontiers in insect science · 2026Article
- An environmental risk assessment of maize containing event, DP-Ø51291-2, with activity against corn rootworms (GM crops & food · 2025Article
- Effective target genes for RNA interference-based management of the cabbage stem flea beetle.Insect molecular biology · 2025Article
- Toxicological and Functional Assessment of Minicell-Encapsulated dsRNA on Biocontrol Agents in Agriculture.ACS environmental Au · 2025Article
- Sec61s and Sec62/Sec63 Genes Are Essential for Survival by Regulating the Gut and Cuticle Development inInsects · 2025Article
- Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.Pest management science · 2025Article
- Spray-induced gene silencing for crop protection: recent advances and emerging trends.Frontiers in plant science · 2025Review
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- Nanocarrier-Based Eco-Friendly RNA Pesticides for Sustainable Management of Plant Pathogens and Pests.Nanomaterials (Basel, Switzerland) · 2024Review
- RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa.Scientific reports · 2024Article
- Article
- Control of two insect pests by expression of a mismatch corrected double-stranded RNA in plants.Plant biotechnology journal · 2024Article
- The role of polyplexes in developing a green sustainable approach in agriculture.RSC advances · 2022Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sequence specificity of the endogenous RNA interference pathway allows targeted suppression of genes essential for insect survival and enables the development of durable and efficacious insecticidal products having a low likelihood to adversely impact non-target organisms. The spectrum of insecticidal activity of a 240 nucleotide (nt) dsRNA targeting the Snf7 ortholog in Western Corn Rootworm (WCR; Diabrotica virgifera virgifera) was characterized by selecting and testing insects based upon their phylogenetic relatedness to WCR. Insect species, representing 10 families and 4 Orders, were evaluated in subchronic or chronic diet bioassays that measured potential lethal and sublethal effects. When a specific species could not be tested in diet bioassays, the ortholog to the WCR Snf7 gene (DvSnf7) was cloned and corresponding dsRNAs were tested against WCR and Colorado potato beetle (Leptinotarsa decemlineata); model systems known to be sensitive to ingested dsRNA. Bioassay results demonstrate that the spectrum of activity for DvSnf7 is narrow and activity is only evident in a subset of beetles within the Galerucinae subfamily of Chrysomelidae (>90% identity with WCR Snf7 240 nt). This approach allowed for evaluating the relationship between minimum shared nt sequence length and activity. A shared sequence length of ≥ 21 nt was required for efficacy against WCR (containing 221 potential 21-nt matches) and all active orthologs contained at least three 21 nt matches. These results also suggest that WCR resistance to DvSnf7 dsRNA due to single nucleotide polymorphisms in the target sequence of 240 nt is highly unlikely.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.