ArticleIranian biomedical journal2013
Colony forming unit endothelial cells do not exhibit telomerase alternative splicing variants and activity.
Article in Iranian biomedical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- AICAR and nicotinamide treatment synergistically augment the proliferation and attenuate senescence-associated changes in mesenchymal stromal cells.Stem cell research & therapy · 2020Article
- Expression Pattern of Telomerase Reverse Transcriptase (hTERT) Variants and Bcl-2 in Peripheral Lymphocytes of Systemic Lupus Erythematosus Patients.Iranian journal of pathology · 2020Article
- Association of coronary artery dominance and mortality rate and complications in patients with ST-segment elevation myocardial infarction treated with primary percutaneous coronary intervention.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2020Article
- Global longitudinal strain as an Indicator of cardiac Iron overload in thalassemia patients.Cardiovascular ultrasound · 2019Article
- Reduced myocardial reserve in cirrhotic patients: an evaluation by dobutamine stress speckle tracking and tissue Doppler imaging (TDI) echocardiography.Journal of cardiovascular and thoracic research · 2019Article
- Purification of Stem Cells from Oral Pyogenic Granuloma Tissue.The open dentistry journal · 2018Article
- Low Dose Spironolactone Monotherapy in the Management of Stage I Essential Hypertension: A Pilot Randomized, Double-Blind, Placebo-Controlled Trial.Acta Cardiologica Sinica · 2018Article
- Role of CD146 Enrichment in Purification of Stem Cells Derived from Dental Pulp Polyp.Iranian endodontic journal · 2017Article
- Identification of Mesenchymal Stem Cell Marker STRO-1 in Oral Reactive Lesions by Immunofluorescence Method.Journal of dentistry (Shiraz, Iran) · 2015Article
- Absence of Correlation between Changes in the Number of Endothelial Progenitor Cell Subsets.Korean circulation journal · 2015Article
- The association of plasma high-sensitivity C-reactive protein level with rheumatic heart disease: The possible role of inflammation.Indian heart journalArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionEndothelial progenitor colony forming unit-endothelial cells (CFU-EC) were first believed to be the progenitors of endothelial cells, named endothelial progenitor cells. Further studies revealed that they are monocytes regulating vasculogenesis. The main hindrance of these cells for therapeutic purposes is their low frequency and limited replicative potentials. This study was undertaken to determine telomerase activity and alternative splicing variants in CFU-EC as a potential cause of limited replicative capacity in these cells.
methodsCFU-EC were isolated from peripheral blood using a standard cell culture assay. Colonies were detached mechanically and alternative splicing variant mRNA were evaluated using real-time PCR. Telomerase enzyme activity was assessed using telomerase repeat amplification protocol. The same procedures were done on the cancer cell line Calu6 as the positive control.
resultsThe cultured peripheral blood mononuclear cells formed colonies with spindle-shaped monocytic cells sprouted from the clusters. These morphological characteristics fulfill the definition of CFU-EC. Telomere length amplification protocol assay revealed no telomerase activity and real-time PCR showed no expression of telomerase enzyme mRNA in CFU-EC. Both parameters were significantly higher in the cancer cell line Calu6 taken as the positive control.
conclusionThe absence of telomerase activity in the CFU-EC is a result of pre-transcriptional regulation of gene expression rather than other mechanisms for controlling telomerase activity such as post-transcriptional modifications. This finding can explain the limited proliferative activity of CFU-EC cells. We propose that absence of telomerase activity in CFU-EC can be attributable to their more mature monocytic nature that needs further investigations.
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