ArticleNucleic acids research2013
Investigation of DNA sequence recognition by a streptomycete MarR family transcriptional regulator through surface plasmon resonance and X-ray crystallography.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- TagR, a newly identified member of the MarR family of transcriptional regulators, represses the NRPS operon inMicrobiology spectrum · 2026Article
- Redox control of antibiotic biosynthesis.mBio · 2025Article
- ArnR binds a [4Fe-4S] cluster and indirectly senses anaerobicity in Corynebacteria.Metallomics : integrated biometal science · 2025Article
- The TetR-like regulator Sco4385 and Crp-like regulator Sco3571 modulate heterologous production of antibiotics inApplied and environmental microbiology · 2025Article
- Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix-turn-helix protein RdfS.Nucleic acids research · 2025Article
- Stabilisation of the RirA [4Fe-4S] cluster results in loss of iron-sensing function.Chemical science · 2023Article
- Global Effects of the Developmental Regulator BldB in Streptomyces venezuelae.Journal of bacteriology · 2023Article
- Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.PLoS biology · 2023Article
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances inInternational journal of molecular sciences · 2022Article
- Prophage-like gene transfer agents promote Caulobacter crescentus survival and DNA repair during stationary phase.PLoS biology · 2022Article
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- An epigenetic switch activates bacterial quorum sensing and horizontal transfer of an integrative and conjugative element.Nucleic acids research · 2022Article
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- Perception of structurally distinct effectors by the integrated WRKY domain of a plant immune receptor.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Evolving origin-of-transfer sequences on staphylococcal conjugative and mobilizable plasmids-who's mimicking whom?Nucleic acids research · 2021Article
- Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- The evolution of MarR family transcription factors as counter-silencers in regulatory networks.Current opinion in microbiology · 2020Review
- The crystal structure of AcrR from Mycobacterium tuberculosis reveals a one-component transcriptional regulation mechanism.FEBS open bio · 2019Article
- Functional duplication of Rap1 in methylotrophic yeasts.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Consistent with their complex lifestyles and rich secondary metabolite profiles, the genomes of streptomycetes encode a plethora of transcription factors, the vast majority of which are uncharacterized. Herein, we use Surface Plasmon Resonance (SPR) to identify and delineate putative operator sites for SCO3205, a MarR family transcriptional regulator from Streptomyces coelicolor that is well represented in sequenced actinomycete genomes. In particular, we use a novel SPR footprinting approach that exploits indirect ligand capture to vastly extend the lifetime of a standard streptavidin SPR chip. We define two operator sites upstream of sco3205 and a pseudopalindromic consensus sequence derived from these enables further potential operator sites to be identified in the S. coelicolor genome. We evaluate each of these through SPR and test the importance of the conserved bases within the consensus sequence. Informed by these results, we determine the crystal structure of a SCO3205-DNA complex at 2.8 Å resolution, enabling molecular level rationalization of the SPR data. Taken together, our observations support a DNA recognition mechanism involving both direct and indirect sequence readout.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.