ArticleJournal of visualized experiments : JoVE2013
Electroporation of the hindbrain to trace axonal trajectories and synaptic targets in the chick embryo.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- Hindbrain boundaries as niches of neural progenitor and stem cells regulated by the extracellular matrix proteoglycan chondroitin sulphate.Development (Cambridge, England) · 2024Article
- Axonal Projection Patterns of the Dorsal Interneuron Populations in the Embryonic Hindbrain.Frontiers in neuroanatomy · 2021Review
- Temporal-specific roles of fragile X mental retardation protein in the development of the hindbrain auditory circuit.Development (Cambridge, England) · 2020Article
- Neural stem cells deriving from chick embryonic hindbrain recapitulate hindbrain development in culture.Scientific reports · 2018Article
- Control of axon guidance and neurotransmitter phenotype of dB1 hindbrain interneurons by Lim-HD code.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electroporation of the chick embryonic neural tube has many advantages such as being quick and efficient for the expression of foreign genes into neuronal cells. In this manuscript we provide a method that demonstrates uniquely how to electroporate DNA into the avian hindbrain at E2.75 in order to specifically label a subset of neuronal progenitors, and how to follow their axonal projections and synaptic targets at much advanced stages of development, up to E14.5. We have utilized novel genetic tools including specific enhancer elements, Cre/Lox - based plasmids and the PiggyBac-mediated DNA transposition system to drive GFP expression in a subtype of hindbrain cells (the dorsal most subgroup of interneurons, dA1). Axonal trajectories and targets of dA1 axons are followed at early and late embryonic stages at various brainstem regions. This strategy contributes advanced techniques for targeting cells of interest in the embryonic hindbrain and for tracing circuit formation at multiple stages of development.
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Registered trials
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