ArticleEukaryotic cell2013
Spatial reorganization of Saccharomyces cerevisiae enolase to alter carbon metabolism under hypoxia.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Short autoinhibitory sequences control phase separation of an essential bacterial transcription termination factor.The EMBO journal · 2026Article
- Cellular view of metabolism: metabolic biomolecular condensates.The New phytologist · 2025Review
- TPM4 condensates glycolytic enzymes and facilitates actin reorganization under hyperosmotic stress.Cell discovery · 2024Article
- Organization of a functional glycolytic metabolon on mitochondria for metabolic efficiency.Nature metabolism · 2024Article
- Hypoxia-inducible factor-driven glycolytic adaptations in host-microbe interactions.Pflugers Archiv : European journal of physiology · 2024Review
- Protein aggregation and biomolecular condensation in hypoxic environments (Review).International journal of molecular medicine · 2024Review
- Hypoxia induces a glycolytic complex in intestinal epithelial cells independent of HIF-1-driven glycolytic gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Activation of L-lactate oxidase by the formation of enzyme assemblies through liquid-liquid phase separation.Scientific reports · 2023Article
- Article
- Biomolecular Condensation: A New Phase in Cancer Research.Cancer discovery · 2022Review
- Condensate Formation by Metabolic Enzymes inMicroorganisms · 2022Review
- Micellization: A new principle in the formation of biomolecular condensates.Frontiers in molecular biosciences · 2022Review
- Compartmentalization and metabolic regulation of glycolysis.Journal of cell science · 2021Review
- Phosphofructokinase relocalizes into subcellular compartments with liquid-like properties in vivo.Biophysical journal · 2021Article
- Transcriptomic Insights into the Effect of Melatonin inAntioxidants (Basel, Switzerland) · 2020Article
- Hypoxia drives the assembly of the multienzyme purinosome complex.The Journal of biological chemistry · 2020Article
- Article
- Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation.Biophysical reviews · 2019Review
- Defining the RNA interactome by total RNA-associated protein purification.Molecular systems biology · 2019Article
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7 authors.
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Abstract
Hypoxia has critical effects on the physiology of organisms. In the yeast Saccharomyces cerevisiae, glycolytic enzymes, including enolase (Eno2p), formed cellular foci under hypoxia. Here, we investigated the regulation and biological functions of these foci. Focus formation by Eno2p was inhibited temperature independently by the addition of cycloheximide or rapamycin or by the single substitution of alanine for the Val22 residue. Using mitochondrial inhibitors and an antioxidant, mitochondrial reactive oxygen species (ROS) production was shown to participate in focus formation. Focus formation was also inhibited temperature dependently by an SNF1 knockout mutation. Interestingly, the foci were observed in the cell even after reoxygenation. The metabolic turnover analysis revealed that [U-(13)C]glucose conversion to pyruvate and oxaloacetate was accelerated in focus-forming cells. These results suggest that under hypoxia, S. cerevisiae cells sense mitochondrial ROS and, by the involvement of SNF1/AMPK, spatially reorganize metabolic enzymes in the cytosol via de novo protein synthesis, which subsequently increases carbon metabolism. The mechanism may be important for yeast cells under hypoxia, to quickly provide both energy and substrates for the biosynthesis of lipids and proteins independently of the tricarboxylic acid (TCA) cycle and also to fit changing environments.
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