ArticleAnalytical biochemistry2013
Discrepancy between fluorescence correlation spectroscopy and fluorescence recovery after photobleaching diffusion measurements of G-protein-coupled receptors.
Article in Analytical biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Characterising C-X-C Chemokine Receptor 4 Dynamics in the Cell Membrane Using Fluorescence Fluctuation Spectroscopy.Biomolecules · 2026Article
- Imaging methods to monitor and quantify cell differentiation.Frontiers in cell and developmental biology · 2025Review
- Experimentally Probing the Effect of Confinement Geometry on Lipid Diffusion.The journal of physical chemistry. B · 2024Article
- Antimicrobial Photodynamic Inactivation Using Topical and Superhydrophobic Sensitizer Techniques: A Perspective from Diffusion in Biofilms.Photochemistry and photobiology · 2021Review
- Hydrogels as Drug Delivery Systems: A Review of Current Characterization and Evaluation Techniques.Pharmaceutics · 2020Review
- Temporal dependence of shifts in mu opioid receptor mobility at the cell surface after agonist binding observed by single-particle tracking.Scientific reports · 2019Article
- SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules.Biophysical journal · 2018Article
- High-Speed Single-Molecule Tracking of CXCL13 in the B-Follicle.Frontiers in immunology · 2018Article
- Cell shape information is transduced through tension-independent mechanisms.Nature communications · 2017Article
- Disruption of Ankyrin B and Caveolin-1 Interaction Sites Alters NaBiophysical journal · 2017Article
- Super-resolution Visualization of Caveola Deformation in Response to Osmotic Stress.The Journal of biological chemistry · 2017Article
- On the Equivalence of FCS and FRAP: Simultaneous Lipid Membrane Measurements.Biophysical journal · 2016Article
- Osmotic Stress Reduces Ca2+ Signals through Deformation of Caveolae.The Journal of biological chemistry · 2015Article
- Heterologous regulation of Mu-opioid (MOP) receptor mobility in the membrane of SH-SY5Y cells.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) are the two most direct methods to measure the diffusion of molecules in intact living cells. Ideally, these methods should produce similar results for an identical system. We have used these methods to monitor the diffusion of two G-protein-coupled receptors and their associated proteins in the plasma membranes of cells that do not or do contain invaginated protein domains called caveolae. FRAP studies show that caveolae domains increase the immobile fraction of receptors without significantly changing their mobility. On the other hand, FCS studies show an unexpected increase the mobility of caveolae-associated proteins. Our data suggest that the geometry of caveolae domains gives rise to a confined diffusion of its attached proteins, resulting in an apparent increase in mobility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.