ArticleMatrix biology : journal of the International Society for Matrix Biology
Leucine rich amelogenin peptide alters ameloblast differentiation in vivo.
Article in Matrix biology : journal of the International Society for Matrix Biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs.Medical molecular morphology · 2026Article
- A splicing-derived microRNA from amelogenin exon4 regulates enamel formation via control of exon4 splicing and amelogenin expression.Scientific reports · 2026Article
- Polarized, Amelogenin Expressing Ameloblast-Like Cells from Cervical Loop/Dental Pulp Cocultures in Bioreactors.Stem cells and development · 2021Article
- Transcriptional Regulation of Dental Epithelial Cell Fate.International journal of molecular sciences · 2020Review
- Tooth Enamel and its Dynamic Protein Matrix.International journal of molecular sciences · 2020Review
- Expression of amelogenin and effects of cyclosporin A in developing hair follicles in rats.Journal of anatomy · 2016Article
- Exon4 amelogenin transcripts in enamel biomineralization.Journal of dental research · 2015Article
- The leucine-rich amelogenin protein (LRAP) is primarily monomeric and unstructured in physiological solution.Journal of structural biology · 2015Article
- MSX2 in ameloblast cell fate and activity.Frontiers in physiology · 2014Review
- Tracking endogenous amelogenin and ameloblastin in vivo.PloS one · 2014Article
- The flexible structure of the K24S28 region of Leucine-Rich Amelogenin Protein (LRAP) bound to apatites as a function of surface type, calcium, mutation, and ionic strength.Frontiers in physiology · 2014Article
- Analysis of enamel development using murine model systems: approaches and limitations.Frontiers in physiology · 2014Review
- Truncated amelogenin and LRAP transgenes improve Amelx null mouse enamel.Matrix biology : journal of the International Society for Matrix BiologyArticle
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Highly mineralized tooth enamel develops from an extracellular matrix chiefly comprised of amelogenins formed by splicing of 7 (human) or 9 (rodent) exons secreted from specialized epithelial cells known as ameloblasts. Here we examined the role of the 59 amino acid alternatively spliced amelogenin known as leucine rich amelogenin peptide (LRAP) on enamel formation, using transgenic murine models in which LRAP overexpression is driven by an amelogenin promoter (TgLRAP). Beginning in the secretory stage of mouse amelogenesis, we found a reduced thickness of enamel matrix and a loss of Tomes' processes, followed by upregulated amelogenin mRNA expression, inhibited amelogenin secretion and loss of cell polarity. In the presecretory stage (P0) amelogenin m180 mRNA expression was increased 58 fold along with a 203 fold increase in MMP-20 expression and 3.5 and 3.2 fold increased in respectively enamelin and ameloblastin. When LRAP was overexpressed on an amelogenin knockout mouse model, the ameloblasts were not affected. Further, expression of the global chromatin organizer and transcription factor SATB1 was reduced in secretory stage TgLRAP ameloblasts. These findings identify a cellular role for LRAP in enamel formation that is not directly related to directing enamel crystal formation as is reported to be the primary function of full length amelogenins. The effect of LRAP overexpression in upregulating amelogenins, MMP-20 and SATB1, suggests a role in protein regulation critical to ameloblast secretion and matrix processing, to form a mineralized enamel matrix.
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