ArticleApplied and environmental microbiology2013
Bacterial cell wall synthesis gene uppP is required for Burkholderia colonization of the Stinkbug Gut.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- InferiorMicrobial genomics · 2024Pooled it
- Leaffooted bugs enrich local soil with their horizontally acquired symbiont.Frontiers in microbiology · 2026Article
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- Single-step genome engineering in the bee gut symbiontbioRxiv : the preprint server for biology · 2023Article
- Proteolytic Activity of DegP Is Required for theMicrobiology spectrum · 2023Article
- How It All Begins: Bacterial Factors Mediating the Colonization of Invertebrate Hosts by Beneficial Symbionts.Microbiology and molecular biology reviews : MMBR · 2022Review
- Prevalence of an Insect-Associated Genomic Region in Environmentally AcquiredApplied and environmental microbiology · 2022Article
- Impact of Peptidoglycan Recycling Blockade and Expression of Horizontally Acquired β-Lactamases on Pseudomonas aeruginosa Virulence.Microbiology spectrum · 2022Article
- Dual oxidase enables insect gut symbiosis by mediating respiratory network formation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Transcriptional Regulatory Network ofMicroorganisms · 2021Review
- Multiple Precursor Proteins of Thanatin Isoforms, an Antimicrobial Peptide Associated With the Gut Symbiont ofFrontiers in microbiology · 2021Article
- The Importance of Environmentally Acquired Bacterial Symbionts for the Squash Bug (Frontiers in microbiology · 2021Article
- Synergistic Inactivation of Bacteria Using a Combination of Erythorbyl Laurate and UV Type-A Light Treatment.Frontiers in microbiology · 2021Article
- Article
- Host-symbiont specificity determined by microbe-microbe competition in an insect gut.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Comparative cytology, physiology and transcriptomics of Burkholderia insecticola in symbiosis with the bean bug Riptortus pedestris and in culture.The ISME journal · 2019Article
- Profiling the susceptibility of Pseudomonas aeruginosa strains from acute and chronic infections to cell-wall-targeting immune proteins.Scientific reports · 2019Article
- Interplay between Peptidoglycan Biology and Virulence in Gram-Negative Pathogens.Microbiology and molecular biology reviews : MMBR · 2018Review
- Infection dynamics of insecticide-degrading symbionts from soil to insects in response to insecticide spraying.The ISME journal · 2018Article
- The lipopolysaccharide core oligosaccharide ofThe Journal of biological chemistry · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To establish a host-bacterium symbiotic association, a number of factors involved in symbiosis must operate in a coordinated manner. In insects, bacterial factors for symbiosis have been poorly characterized at the molecular and biochemical levels, since many symbionts have not yet been cultured or are as yet genetically intractable. Recently, the symbiotic association between a stinkbug, Riptortus pedestris, and its beneficial gut bacterium, Burkholderia sp., has emerged as a promising experimental model system, providing opportunities to study insect symbiosis using genetically manipulated symbiotic bacteria. Here, in search of bacterial symbiotic factors, we targeted cell wall components of the Burkholderia symbiont by disruption of uppP gene, which encodes undecaprenyl pyrophosphate phosphatase involved in biosynthesis of various bacterial cell wall components. Under culture conditions, the ΔuppP mutant showed higher susceptibility to lysozyme than the wild-type strain, indicating impaired integrity of peptidoglycan of the mutant. When administered to the host insect, the ΔuppP mutant failed to establish normal symbiotic association: the bacterial cells reached to the symbiotic midgut but neither proliferated nor persisted there. Transformation of the ΔuppP mutant with uppP-encoding plasmid complemented these phenotypic defects: lysozyme susceptibility in vitro was restored, and normal infection and proliferation in the midgut symbiotic organ were observed in vivo. The ΔuppP mutant also exhibited susceptibility to hypotonic, hypertonic, and centrifugal stresses. These results suggest that peptidoglycan cell wall integrity is a stress resistance factor relevant to the successful colonization of the stinkbug midgut by Burkholderia symbiont.
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