ArticleApplied and environmental microbiology2013
Identification and characterization of a novel flagellum-dependent Salmonella-infecting bacteriophage, iEPS5.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed.
- Analysis of bacteriophage diversity in Ganga river ecosystem: insights from metagenome-based sequencing.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Battle beyond membrane: flagella as a conduit for phage DNA entry and a trigger for bacterial defense in Yersinia enterocolitica.Nucleic acids research · 2025Article
- A machine learning approach to predict strain-specific phage-host interactions.Scientific reports · 2025Article
- A flagella-dependentmBio · 2025Article
- Deciphering the adsorption machinery of Deep-Blue and Vp4, two myophages targeting members of theJournal of virology · 2024Article
- Prevalence of antibiotic-resistantFood science and biotechnology · 2024Article
- A hybrid receptor binding protein enables phage F341 infection ofFrontiers in microbiology · 2024Article
- Targeted dual-receptor phage cocktail againstFrontiers in microbiology · 2024Article
- Phage Therapy, a Salvage Treatment for Multidrug-Resistant Bacteria Causing Infective Endocarditis.Biomedicines · 2023Review
- Dominance of phage particles carrying antibiotic resistance genes in the viromes of retail food sources.The ISME journal · 2023Article
- Phage Adsorption to Gram-Positive Bacteria.Viruses · 2023Review
- Isolation and Characterization of Chi-like Salmonella Bacteriophages Infecting Two Salmonella enterica Serovars, Typhimurium and EnteritidisPathogens (Basel, Switzerland) · 2022Article
- Comparative transcriptome combined with morphophysiological analyses revealed the molecular mechanism underlyingVirulence · 2022Article
- Development of a Bacteriophage Cocktail against Pectobacterium carotovorum Subsp.Applied and environmental microbiology · 2022Article
- Biological Features and In Planta Transcriptomic Analyses of aInternational journal of molecular sciences · 2022Article
- Flagellotropic Bacteriophages: Opportunities and Challenges for Antimicrobial Applications.International journal of molecular sciences · 2022Review
- Complete Genome Sequences of Six Chi-Like Bacteriophages That Infect Proteus and Klebsiella.Microbiology resource announcements · 2022Article
- Mechanisms and clinical importance of bacteriophage resistance.FEMS microbiology reviews · 2022Review
- Article
- Isolation and Characterisation of Bacteriophages with Activity against Invasive Non-TyphoidalViruses · 2021Article
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Authors and funding
4 authors.
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Abstract
A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the receptor for iEPS5, Tn5 transposon insertion mutants of S. Typhimurium SL1344 that were resistant to the phage were isolated. All of the phage-resistant mutants were found to have mutations in genes involved in flagellar formation, suggesting that the flagellum is the adsorption target of this phage. Analysis of phage infection using the ΔmotA mutant, which is flagellated but nonmotile, demonstrated the requirement of flagellar rotation for iEPS5 infection. Further analysis of phage infection using the ΔcheY mutant revealed that iEPS5 could infect host bacteria only when the flagellum is rotating counterclockwise (CCW). These results suggested that the CCW-rotating flagellar filament is essential for phage adsorption and required for successful infection by iEPS5. In contrast to the well-studied flagellatropic phage Chi, iEPS5 cannot infect the ΔfliK mutant that makes a polyhook without a flagellar filament, suggesting that these two flagellatropic phages utilize different infection mechanisms. Here, we present evidence that iEPS5 injects its DNA into the flagellar filament for infection by assessing DNA transfer from SYBR gold-labeled iEPS5 to the host bacteria.
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