Evidence map›Paper›PMID 23747294›Full record

ArticleBiochimica et biophysica acta2013

Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.

Tatyana M Konyakhina, Jing Wu, James D Mastroianni, Frederick A Heberle, Gerald W Feigenson

Abstract read
In one paragraph

Article in Biochimica et biophysica acta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Article
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  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Experimentally determined leaflet-leaflet phase diagram of an asymmetric lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  10. Article
  11. Review
  12. Article
  13. Recombination betweenAnalytical chemistry · 2022
    Article
  14. Yeast cells actively tune their membranes to phase separate at temperatures that scale with growth temperatures.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  15. Article
  16. Article
  17. Critical Phenomena in Plasma Membrane Organization and Function.Annual review of physical chemistry · 2021
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tatyana M KonyakhinaDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Jing Wu
James D Mastroianni
Frederick A Heberle
Gerald W Feigenson

Funding

TRAINING IN MOLECULAR PHYSICS OF BIOLOGICAL SYSTEMST32GM008267 · NIGMS · CORNELL UNIVERSITY ITHACA · PI WANG, MICHELLE D. · 1988 to 2021
$9.6M
Membrane Control of Protein-Protein Contact. Simulation Based on Phase DiagramsR01GM077198 · NIGMS · CORNELL UNIVERSITY · PI FEIGENSON, GERALD WILLIAM · 2007 to 2010
$1.6M
NIGMS NIH HHS 1-T32-GM08267NIGMS NIH HHS R01 GM077198NIGMS NIH HHS T32 GM008267
6 · The paper itself

Abstract

We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld+Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld+Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.

Indexed as

CholesterolFluorescence Resonance Energy TransferPhase TransitionPhosphatidylcholines1,2-distearoyllecithin1,2-oleoylphosphatidylcholine1-palmitoyl-2-oleoylphosphatidylcholineCholesterolPhosphatidylcholines1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate1,1′-dieicosanyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate1,2-Dilauroyl-sn-glycero-3-phosphocholine1,2-Dioleoyl-sn-glycero-3-phosphocholine1,2-Dipalmitoyl-sn-glycero-3-phosphocholine1,2-Distearoyl-sn-glycero-3-phosphocholine1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine1-Stearoyl-2-oleoyl-sn-glycero-3-phosphocholine2-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine3-Dye method4-Component lipid phase diagramBoDIPY-PCC12:0-DiIC20:0-DiICholCholesterolCompeting interactionDHEDLPCDOPCDPPCDSPCErgosta-5,7,9(11),22-tetraen-3β-olFörster resonance energy transferFRETGiant unilamellar vesicleGUVLeft hand side refers to left side of phase diagram, i.e. at lower χ(DSPC)LHSLipid raftModulated phasePCPhosphatidylcholinePOPCQuaternary phase diagramRapid solvent exchangeREERegion of enhanced efficiencyRegion of reduced efficiencyRHSRight hand side, refers to right side of phase diagram, i.e.,, at higher χ(DSPC)RRERSESAESensitized acceptor emissionSMSOPCSphingomyelinT1–T6Thin-layer chromatographyTLCTOETrajectories 1–6. bSM, sphingomyelin derived from porcine brainTrp-Oleoyl Ester, N-oleoyl-dl-tryptophan ethyl ester

Identifiers

PMID23747294
PMCPMC3738200

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.