ArticleBiomaterials2013
Neuroglial differentiation of adult enteric neuronal progenitor cells as a function of extracellular matrix composition.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
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- Persistent enteric neuroinflammation chronically impairs colonic motility in a pyridostigmine bromide-induced mouse model of Gulf War illness.Biology open · 2025Article
- Cecal growth factors promote enteric neurosphere formation and hindgut colonization in the avian model.Frontiers in cell and developmental biology · 2025Article
- Tissue engineering and regenerative medicine approaches in colorectal surgery.Annals of coloproctology · 2024Review
- Crisis in the gut: navigating gastrointestinal challenges in Gulf War Illness with bioengineering.Military Medical Research · 2024Review
- Extracellular matrix substrates differentially influence enteric glial cell homeostasis and immune reactivity.Frontiers in immunology · 2024Article
- Hirschsprung disease.Nature reviews. Disease primers · 2023Review
- Development of Brain-Derived Bioscaffolds for Neural Progenitor Cell Culture.ACS pharmacology & translational science · 2023Article
- Association of mast-cell-related conditions with hypermobile syndromes: a review of the literature.Immunologic research · 2022Review
- Surgical Treatment of Short Bowel Syndrome-The Past, the Present and the Future, a Descriptive Review of the Literature.Children (Basel, Switzerland) · 2022Review
- The gut brain in a dish: Murine primary enteric nervous system cell cultures.Neurogastroenterology and motility · 2022Review
- CD93 negatively regulates astrogenesis in response to MMRN2 through the transcriptional repressor ZFP503 in the developing brain.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells.Iranian journal of basic medical sciences · 2020Article
- Bioengineering and regeneration of gastrointestinal tissue: where are we now and what comes next?Expert opinion on biological therapy · 2019Review
- Laminin and Environmental Cues Act in the Inhibition of the Neuronal Differentiation of Enteric GliaFrontiers in neuroscience · 2019Article
- Enhancement of Neural Stem Cell Survival, Proliferation, Migration, and Differentiation in a Novel Self-Assembly Peptide Nanofibber Scaffold.Molecular neurobiology · 2017Article
- Identification of a rare LAMB4 variant associated with familial diverticulitis through exome sequencing.Human molecular genetics · 2017Article
- Bioengineering the gut: future prospects of regenerative medicine.Nature reviews. Gastroenterology & hepatology · 2016Review
- Bioengineering functional human sphincteric and non-sphincteric gastrointestinal smooth muscle constructs.Methods (San Diego, Calif.) · 2016Article
- New approaches to increase intestinal length: Methods used for intestinal regeneration and bioengineering.World journal of transplantation · 2016Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Enteric neuronal progenitor cells are neural crest-derived stem cells that can be isolated from fetal, post-natal and adult gut. Neural stem cell transplantation is an emerging therapeutic paradigm to replace dysfunctional or lost enteric neurons in several aganglionic disorders of the GI tract. The impetus to identify an appropriate microenvironment for enteric neuronal progenitor cells derives from the need to improve survival and phenotypic stability following implantation. Extracellular matrix composition can modulate stem cell fate and direct differentiation. Adult mammalian myenteric ganglia in vivo are surrounded by a matrix composed primarily of Collagen IV, Laminin and a Heparan sulfate proteoglycan. In these studies, adult mammalian enteric neuronal progenitor cells isolated from full thickness rabbit intestines were induced to differentiate when cultured on various combinations of neural ECM substrates. Neuronal and glial differentiation was studied as a function of ECM composition on coated glass coverslips. Poly-lysine coated coverslips (control) supported extensive glial differentiation but very minimal neuronal differentiation. Individual culture substrata (Laminin, Collagen I and Collagen IV) were conducive for both neuronal and glial differentiation. The addition of laminin or heparan sulfate to collagen substrates improved neuronal differentiation, significantly increased neurite lengths, branching and initiation of neuronal network formation. Glial differentiation was extensive on control poly lysine coated coverslips. Addition of laminin or heparan sulfate to composite collagen substrates significantly reduced glial immunofluorescence. Various neural ECM components were evaluated individually and in combination to study their effect of neuroglial differentiation of adult enteric neuronal progenitor cells. Our results indicate that specific ECM substrates that include type IV Collagen, laminin and heparan sulfate support and maintain neuronal and glial differentiation to different extents. Here, we identify a matrix composition optimized to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.