ArticleCell reports2013
Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation.
Article in Cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 40 citations in OpenAlex.
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- Retention of duplicated genes in evolution.Trends in genetics : TIG · 2022Review
- τ-SGA: synthetic genetic array analysis for systematically screening and quantifying trigenic interactions in yeast.Nature protocols · 2021Article
- Genetic interaction mapping informs integrative structure determination of protein complexes.Science (New York, N.Y.) · 2020Article
- CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.PLoS genetics · 2020Article
- Highly Combinatorial Genetic Interaction Analysis Reveals a Multi-Drug Transporter Influence Network.Cell systems · 2020Article
- Synthetic ablations in theNetwork neuroscience (Cambridge, Mass.) · 2020Article
- Noise-precision tradeoff in predicting combinations of mutations and drugs.PLoS computational biology · 2019Article
- Yeast genetic interaction screens in the age of CRISPR/Cas.Current genetics · 2019Review
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- A genetic approach to study polyubiquitination in Saccharomyces cerevisiae.Methods in enzymology · 2019Article
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- Systematic analysis of complex genetic interactions.Science (New York, N.Y.) · 2018Article
- High-Throughput Quantitative Genetic Interaction Mapping in the Fission YeastCold Spring Harbor protocols · 2018Article
- Mapping a diversity of genetic interactions in yeast.Current opinion in systems biology · 2017Article
- Synthetic lethality and cancer.Nature reviews. Genetics · 2017Review
- Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.Scientific reports · 2017Article
- Article
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
Abstract
Genetic interactions reveal the functional relationships between pairs of genes. In this study, we describe a method for the systematic generation and quantitation of triple mutants, termed triple-mutant analysis (TMA). We have used this approach to interrogate partially redundant pairs of genes in S. cerevisiae, including ASF1 and CAC1, two histone chaperones. After subjecting asf1Δ cac1Δ to TMA, we found that the Swi/Snf Rdh54 protein compensates for the absence of Asf1 and Cac1. Rdh54 more strongly associates with the chromatin apparatus and the pericentromeric region in the double mutant. Moreover, Asf1 is responsible for the synthetic lethality observed in cac1Δ strains lacking the HIRA-like proteins. A similar TMA was carried out after deleting both CLB5 and CLB6, cyclins that regulate DNA replication, revealing a strong functional connection to chromosome segregation. This approach can reveal functional redundancies that cannot be uncovered through traditional double-mutant analyses.
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