ArticleFrontiers in microbiology2013
Fluoroquinolone resistance mechanisms in an Escherichia coli isolate, HUE1, without quinolone resistance-determining region mutations.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 54 citations in OpenAlex.
- Antibiotics: From Mechanism of Action to Resistance and Beyond.Indian journal of microbiology · 2024Review
- Coexistence of plasmid-mediated quinolone resistance (PMQR) and extended-spectrum beta-lactamase (ESBL) genes among clinical Pseudomonas aeruginosa isolates in Egypt.BMC microbiology · 2024Article
- High prevalence of plasmid-mediated quinolone resistance in escherichia coli strains producing extended-spectrum beta-lactamases isolated from faeces and urine of pregnant women with acute cystitis.Molecular biology reports · 2024Article
- UropathogenicMicroorganisms · 2023Review
- Fluoroquinolone resistance contributing mechanisms and genotypes of ciprofloxacin- unsusceptible Pseudomonas aeruginosa strains in Iran: emergence of isolates carrying qnr/aac(6)-Ib genes.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Multidrug Resistance Pumps as a Keystone of Bacterial Resistance.Moscow University biological sciences bulletin · 2022Article
- Expression of the qepA1 gene is induced under antibiotic exposure.The Journal of antimicrobial chemotherapy · 2021Article
- Limited Multidrug Resistance Efflux Pump Overexpression among Multidrug-Resistant Escherichia coli Strains of ST131.Antimicrobial agents and chemotherapy · 2021Article
- Resistance/fitness trade-off is a barrier to the evolution of MarR inactivation mutants in Escherichia coli.The Journal of antimicrobial chemotherapy · 2021Article
- Contribution of Different Mechanisms to Ciprofloxacin Resistance inFrontiers in microbiology · 2021Article
- Whole-Genome Sequence of Fluoroquinolone-Resistant Escherichia coli HUE1, Isolated in Hokkaido, Japan.Microbiology resource announcements · 2020Article
- Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria.Lab on a chip · 2020Article
- Epidemiology and molecular characterization of the antimicrobial resistance ofCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2019Article
- The nature and epidemiology of OqxAB, a multidrug efflux pump.Antimicrobial resistance and infection control · 2019Review
- Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli.The Journal of antimicrobial chemotherapy · 2017Article
- Environmental and genetic modulation of the phenotypic expression of antibiotic resistance.FEMS microbiology reviews · 2017Review
- Tigecycline Nonsusceptibility Occurs Exclusively in Fluoroquinolone-Resistant Escherichia coli Clinical Isolates, Including the Major Multidrug-Resistant Lineages O25b:H4-ST131-H30R and O1-ST648.Antimicrobial agents and chemotherapy · 2017Article
- Biological characterization of the hygrobafilomycin antibiotic JBIR-100 and bioinformatic insights into the hygrolide family of natural products.Bioorganic & medicinal chemistry · 2016Article
- Contribution of target alteration, protection and efflux pump in achieving high ciprofloxacin resistance in Enterobacteriaceae.AMB Express · 2016Article
- Plasmid-mediated quinolone resistance determinants in quinolone-resistant Escherichia coli isolated from patients with bacteremia in a university hospital in Taiwan, 2001-2015.Scientific reports · 2016Article
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9 authors at 3 institutions in 1 country.
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Abstract
Fluoroquinolone resistance can cause major clinical problems. Here, we investigated fluoroquinolone resistance mechanisms in a clinical Escherichia coli isolate, HUE1, which had no mutations quinolone resistance-determining regions (QRDRs) of DNA gyrase and topoisomerase IV. HUE1 demonstrated MICs that exceeded the breakpoints for ciprofloxacin, levofloxacin, and norfloxacin. HUE1 harbored oqxAB and qnrS1 on distinct plasmids. In addition, it exhibited lower intracellular ciprofloxacin concentrations and higher mRNA expression levels of efflux pumps and their global activators than did reference strains. The genes encoding AcrR (local AcrAB repressor) and MarR (MarA repressor) were disrupted by insertion of the transposon IS3-IS629 and a frameshift mutation, respectively. A series of mutants derived from HUE1 were obtained by plasmid curing and gene knockout using homologous recombination. Compared to the MICs of the parent strain HUE1, the fluoroquinolone MICs of these mutants indicated that qnrS1, oqxAB, acrAB, acrF, acrD, mdtK, mdfA, and tolC contributed to the reduced susceptibility to fluoroquinolone in HUE1. Therefore, fluoroquinolone resistance in HUE1 is caused by concomitant acquisition of QnrS1 and OqxAB and overexpression of AcrAB-TolC and other chromosome-encoded efflux pumps. Thus, we have demonstrated that QRDR mutations are not absolutely necessary for acquiring fluoroquinolone resistance in E. coli.
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