ArticleNature protocols2013
Sub-millisecond ligand probing of cell receptors with multiple solution exchange.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Fast functional mapping of ligand-gated ion channels.Communications biology · 2023Article
- Human neutrophils communicate remotely via calcium-dependent glutamate-induced glutamate release.iScience · 2023Article
- Volume-transmitted GABA waves pace epileptiform rhythms in the hippocampal network.Current biology : CB · 2023Article
- Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags.Nature protocols · 2022Review
- Multi-target action of β-alanine protects cerebellar tissue from ischemic damage.Cell death & disease · 2022Article
- The regulatory role of GABAPflugers Archiv : European journal of physiology · 2021Article
- Article
- Extracellular GABA waves regulate coincidence detection in excitatory circuits.The Journal of physiology · 2020Article
- The role of tonic glycinergic conductance in cerebellar granule cell signalling and the effect of gain-of-function mutation.The Journal of physiology · 2019Article
- Biphasic Modulation of NMDA Receptor Function by Metabotropic Glutamate Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Feature Article: Selective modulation of tonically active GABAExperimental biology and medicine (Maywood, N.J.) · 2018Article
- Spontaneously opening GABACell death & disease · 2018Article
- Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.Analytical chemistry · 2017Article
- Moderate AMPA receptor clustering on the nanoscale can efficiently potentiate synaptic current.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The accurate knowledge of receptor kinetics is crucial to our understanding of cell signal transduction in general and neural function in particular. The classical technique of probing membrane receptors on a millisecond scale involves placing a recording micropipette with a membrane patch in front of a double-barrel (θ-glass) application pipette mounted on a piezo actuator. Driven by electric pulses, the actuator can rapidly shift the θ-glass pipette tip, thus exposing the target receptors to alternating ligand solutions. However, membrane patches survive for only a few minutes, thus normally restricting such experiments to a single-application protocol. In order to overcome this deficiency, we have introduced pressurized supply microcircuits in the θ-glass channels, thus enabling repeated replacement of application solutions within 10-15 s. This protocol, which has been validated in our recent studies and takes 20-60 min to implement, allows the characterization of ligand-receptor interactions with high sensitivity, thereby also enabling a powerful paired-sample statistical design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.