ArticleBiochimica et biophysica acta2013
Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes.
Article in Biochimica et biophysica acta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Hypoxia induces robust ATP release from erythrocytes in ApoE-LDLR double-deficient mice.Frontiers in physiology · 2024Article
- Non-Excitatory Amino Acids, Melatonin, and Free Radicals: Examining the Role in Stroke and Aging.Antioxidants (Basel, Switzerland) · 2023Review
- Mechanosensitive Pannexin 1 Activity Is Modulated by Stomatin in Human Red Blood Cells.International journal of molecular sciences · 2022Article
- The broad range di- and tri-nucleotide exchanger SLC35B1 displays asymmetrical affinities for ATP transport across the ER membrane.The Journal of biological chemistry · 2022Article
- Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells Isolated From ApoE/LDLR Double-Deficient Mice.Frontiers in physiology · 2022Article
- Evolution of Mechanical Stress (Archives of Razi Institute · 2021Article
- Pannexin 1 channels control the hemodynamic response to hypoxia by regulating OAmerican journal of physiology. Heart and circulatory physiology · 2021Article
- Generation and Export of Red Blood Cell ATP in Health and Disease.Frontiers in physiology · 2021Review
- Reduced deformability contributes to impaired deoxygenation-induced ATP release from red blood cells of older adult humans.The Journal of physiology · 2019Article
- Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows.International journal of molecular sciences · 2019Article
- Red Blood Cell Deformability, Vasoactive Mediators, and Adhesion.Frontiers in physiology · 2019Review
- Review
- Induction of ATP Release, PPIX Transport, and Cholesterol Uptake by Human Red Blood Cells Using a New Family of TSPO Ligands.International journal of molecular sciences · 2018Article
- Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1.Scientific reports · 2018Article
- Purinergic control of red blood cell metabolism: novel strategies to improve red cell storage quality.Blood transfusion = Trasfusione del sangue · 2017Review
- Article
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Response: Hemolysis is a primary and physiologically relevant ATP release mechanism in human erythrocytes.Blood · 2015Article
- Article
- Regulation of extracellular ATP in human erythrocytes infected with Plasmodium falciparum.PloS one · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe peptide mastoparan 7 (MST7) stimulated ATP release in human erythrocytes. We explored intra- and extracellular processes governing the time-dependent accumulation of extracellular ATP (i.e., ATPe kinetics).
methodsHuman erythrocytes were treated with MST7 in the presence or absence of two blockers of pannexin 1. ATPe concentration was monitored by luciferin-luciferase based real-time luminometry.
resultsExposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling. However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe accumulation was inhibited by 49%. Erythrocytes pre-exposure to 10μM of either carbenoxolone or probenecid, two blockers of pannexin 1, exhibited a partial reduction of ATP efflux. Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice erythrocytes exposed to pannexin 1 blockers.
conclusionsMST7 induced release of ATP required either cell adhesion or strong activation of cAMP synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being fully blocked by pannexin 1 blockers. GENERAL SIGNIFICANCE: Kinetic analysis of extracellular ATP accumulation from human erythrocytes and potential effects on microcirculation.
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