ArticleAmerican journal of respiratory cell and molecular biology2013
H2O2 stimulates cystic fibrosis transmembrane conductance regulator through an autocrine prostaglandin pathway, using multidrug-resistant protein-4.
Article in American journal of respiratory cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Effects of cAMP and CFTR modulation on apical fluid pH in human airway Calu-3 cells.Physiological reports · 2026Article
- Structural basis for substrate and inhibitor recognition of human multidrug transporter MRP4.Communications biology · 2023Article
- Potentiation of long-acting βRespiratory research · 2021Article
- Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells.Scientific reports · 2021Article
- ABCF1 Regulates dsDNA-induced Immune Responses in Human Airway Epithelial Cells.Frontiers in cellular and infection microbiology · 2020Article
- Phosphorylation-dependent modulation of CFTR macromolecular signalling complex activity by cigarette smoke condensate in airway epithelia.Scientific reports · 2019Article
- Ion the Prize: Defining the Complexities of Airway Epithelial Cell Ion Transport Functions.American journal of respiratory cell and molecular biology · 2019Article
- The impact of cigarette smoke exposure, COPD, or asthma status on ABC transporter gene expression in human airway epithelial cells.Scientific reports · 2019Article
- Oxidative stress, autophagy and airway ion transport.American journal of physiology. Cell physiology · 2019Review
- Article
- CFTR supports cell death through ROS-dependent activation of TMEM16F (anoctamin 6).Pflugers Archiv : European journal of physiology · 2018Article
- Cigarette smoke activates CFTR through ROS-stimulated cAMP signaling in human bronchial epithelial cells.American journal of physiology. Cell physiology · 2018Article
- Impaired PGE2-stimulated Cl- and HCO3- secretion contributes to cystic fibrosis airway disease.PloS one · 2017Article
- Article
- Mucosal production of uric acid by airway epithelial cells contributes to particulate matter-induced allergic sensitization.Mucosal immunology · 2016Article
- Dual Oxidase 2 (Duox2) Regulates Pannexin 1-mediated ATP Release in Primary Human Airway Epithelial Cells via Changes in Intracellular pH and Not H2O2 Production.The Journal of biological chemistry · 2016Article
- Soluble adenylyl cyclase mediates hydrogen peroxide-induced changes in epithelial barrier function.Respiratory research · 2016Article
- Hydrogen peroxide stimulation of CFTR reveals an Epac-mediated, soluble AC-dependent cAMP amplification pathway common to GPCR signalling.British journal of pharmacology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cystic fibrosis transmembrane conductance regulator (CFTR) activity is essential for the maintenance of airway surface liquid depth, and therefore mucociliary clearance. Reactive oxygen species, increased during inflammatory airway diseases, alter CFTR activity. Here, H2O2 levels in the surface liquid of normal human bronchial epithelial cultures differentiated at the air-liquid interface were estimated, and H2O2-mediated changes in CFTR activity were examined. In Ussing chambers, H2O2-induced anion currents were sensitive to the CFTR inhibitors CFTRinh172 and GlyH-101. These currents were absent in cells from patients with cystic fibrosis. Responses to greater than 500 μM H2O2 were transient. Cyclooxygenase inhibitors blocked the H2O2 response, as did EP1 and EP4 receptor antagonists. A multidrug-resistant protein (MRP) inhibitor and short hairpin RNA directed against MRP4 blocked H2O2 responses. EP1 and EP4 agonists mimicked H2O2 in both control and MRP4 knockdown cells. Thus, H2O2 activates the synthesis, export, and binding of prostanoids via EP4 and, interestingly, EP1 receptors in normal, differentiated human airway epithelial cells to activate cyclic adenosine monophosphate pathways that in turn activate CFTR channels in the apical membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.