ArticleThe Plant journal : for cell and molecular biology2013
ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3.
Article in The Plant journal : for cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
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Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- Heterologous expression of soybean E3 ubiquitin ligase Glycine max Ring Zinc Finger 1-Like 1 (GmRZFL1) negatively responds to osmotic stress by participating in the abscisic acid signaling pathway in Arabidopsis.BMC plant biology · 2025Article
- BPH1 negatively regulates ABA signaling via AtSAP9 degradation.Plant molecular biology · 2025Article
- Arabidopsis Ubiquitin E3 Ligase AtCHYR1 Promotes ROS Production in Plant Responses to Sugar Availability.Plants (Basel, Switzerland) · 2025Article
- The Pepper E3 Ligase CaGIR1 Acts as a Negative Regulator of Drought Response via Controlling CaGRAS1 Stability.Plant, cell & environment · 2025Article
- Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Review
- Stress-Inducible Expression ofPlants (Basel, Switzerland) · 2024Article
- The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5.The Plant cell · 2024Article
- Apple E3 ligase MdPUB23 mediates ubiquitin-dependent degradation of MdABI5 to delay ABA-triggered leaf senescence.Horticulture research · 2024Article
- Identification and Characterization of thePlants (Basel, Switzerland) · 2024Article
- The 14-3-3 protein OsGF14f interacts with OsbZIP23 and enhances its activity to confer osmotic stress tolerance in rice.The Plant cell · 2023Article
- CRISPR/Cas9 Gene Editing ofInternational journal of molecular sciences · 2022Article
- Regulatory network established by transcription factors transmits drought stress signals in plant.Stress biology · 2022Review
- Characterization and regulation mechanism analysis of ubiquitin-conjugating family genes in strawberry reveals a potential role in fruit ripening.BMC plant biology · 2022Article
- The interaction of ABA and ROS in plant growth and stress resistances.Frontiers in plant science · 2022Review
- IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review.Biology · 2021Review
- Siberian Wildrye (Plants (Basel, Switzerland) · 2021Article
- Factors that affect protein abundance of a positive regulator of abscisic acid signalling, the basic leucine zipper transcription factor ABRE-binding factor 2 (ABF2).Plant direct · 2021Article
- Control of ABA Signaling and Crosstalk with Other Hormones by the Selective Degradation of Pathway Components.International journal of molecular sciences · 2021Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ABA Binding Factor/ABA-Responsive Element Binding Proteins (ABF/AREB) subfamily of bZIP-type transcription factors are positive effectors of ABA responses. Here, we examine the proteolytic regulation of two members: Arabidopsis thaliana ABF1 and ABF3. Both transcription factors are unstable in seedlings, and their degradation is sensitive to proteasome inhibition. ABA treatment of seedlings leads to their rapid accumulation, the result of slowed proteolysis. Deletion of the conserved C-terminal region required for 14-3-3 interaction destabilizes the proteins. The degradation of ABF1 and ABF3 are slower in vivo in seedlings lacking the ubiquitin E3 ligase KEEP ON GOING (KEG), and in vitro in extracts from keg seedlings, implicating KEG in their degradation. ABF1 and ABF3 are ubiquitylation substrates of KEG in vitro, and in vitro pull-down assays document their direct interaction. In contrast to ABI5, another KEG substrate, the degradation of ABFs and proteolytic regulation of ABFs by ABA still occurs in keg seedlings, suggesting that additional E3s participate in ABF1 and ABF3 proteolysis. Loss of ABF1 or ABF3 in the keg background has a phenotypic effect similar to the loss of ABI5, and there is no additional rescue of the keg phenotype in abf1 abf3 abi5 keg seedlings. This result suggests that the abundance of other substrates is altered in keg seedlings, affecting growth. In conclusion, ABF1 and ABF3 abundance is affected by ABA and KEG, and the conserved C4 region serves as a stabilizing element.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.