ArticleeLife2013
TRPM5-mediated calcium uptake regulates mucin secretion from human colon goblet cells.
Article in eLife, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Trial
- LRMDA promotes intestinal mucosal barrier integrity and limits colitis and colon cancer.iScience · 2026Article
- LINC00634 is a novel prognostic biomarker for colon cancer associated with immune infiltration.BMC gastroenterology · 2025Article
- The TRP channels serving as chemical-to-electrical signal converter.Physiological reviews · 2025Review
- Method for Collecting Single Epithelial Cells from the Mouse Larynx.The Laryngoscope · 2024Article
- Tetraspanin-8 sequesters syntaxin-2 to control biphasic release propensity of mucin granules.Nature communications · 2023Article
- "ThermoTRP" Channel Expression in Cancers: Implications for Diagnosis and Prognosis (Practical Approach by a Pathologist).International journal of molecular sciences · 2023Review
- Transient receptor potential channels as predictive marker and potential indicator of chemoresistance in colon cancer.Oncology research · 2023Article
- The ulcerative colitis-associated gene FUT8 regulates the quantity and quality of secreted mucins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins.Nucleic acids research · 2021Article
- Alterations in the CaExploration of targeted anti-tumor therapy · 2021Article
- Mouse intestinal tuft cells express advillin but not villin.Scientific reports · 2020Article
- Biological Redox Impact of Tocopherol Isomers Is Mediated by Fast Cytosolic Calcium Increases in Living Caco-2 Cells.Antioxidants (Basel, Switzerland) · 2020Article
- Dexamethasone Treatment Increases the Intracellular Calcium Level ThroughInternational journal of molecular sciences · 2020Article
- Evidence from ileum and liver transcriptomes of resistance to high-salt and water-deprivation conditions in camel.Zoological letters · 2020Article
- Depletion of the diabetic gut microbiota resistance enhances stem cells therapy in type 1 diabetes mellitus.Theranostics · 2020Article
- Article
- Roles of NaWorld journal of gastroenterology · 2019Review
- Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of CaThe Journal of biological chemistry · 2019Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mucin 5AC (MUC5AC) is secreted by goblet cells of the respiratory tract and, surprisingly, also expressed de novo in mucus secreting cancer lines. siRNA-mediated knockdown of 7343 human gene products in a human colonic cancer goblet cell line (HT29-18N2) revealed new proteins, including a Ca(2+)-activated channel TRPM5, for MUC5AC secretion. TRPM5 was required for PMA and ATP-induced secretion of MUC5AC from the post-Golgi secretory granules. Stable knockdown of TRPM5 reduced a TRPM5-like current and ATP-mediated Ca(2+) signal. ATP-induced MUC5AC secretion depended strongly on Ca(2+) influx, which was markedly reduced in TRPM5 knockdown cells. The difference in ATP-induced Ca(2+) entry between control and TRPM5 knockdown cells was abrogated in the absence of extracellular Ca(2+) and by inhibition of the Na(+)/Ca(2+) exchanger (NCX). Accordingly, MUC5AC secretion was reduced by inhibition of NCX. Thus TRPM5 activation by ATP couples TRPM5-mediated Na(+) entry to promote Ca(2+) uptake via an NCX to trigger MUC5AC secretion. DOI:http://dx.doi.org/10.7554/eLife.00658.001.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.