ArticlePloS one2013
Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Mechanisms of thermoregulation inJournal of bacteriology · 2026Review
- From genome to lifestyle: adaptive strategies ofApplied and environmental microbiology · 2026Article
- Characterization of Hsp17, a Novel Small Heat Shock Protein, in Sphingomonas melonis TY under Heat Stress.Microbiology spectrum · 2023Article
- Temperature Matters: Bacterial Response to Temperature Change.Journal of microbiology (Seoul, Korea) · 2023Review
- Heterologous constitutive production of short-chain-length polyhydroxyalkanoates inFrontiers in bioengineering and biotechnology · 2023Article
- Insights into the temperature responses of Pseudomonas species in beneficial and pathogenic host interactions.Applied microbiology and biotechnology · 2022Review
- Cytoplasmic molecular chaperones in Pseudomonas species.Journal of microbiology (Seoul, Korea) · 2022Review
- Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression.Microbial cell factories · 2022Article
- LeveragingFrontiers in microbiology · 2021Review
- Why? - Successful Pseudomonas aeruginosa clones with a focus on clone C.FEMS microbiology reviews · 2020Review
- An RNA thermometer dictates production of a secreted bacterial toxin.PLoS pathogens · 2020Article
- An unconventional RNA-based thermosensor within the 5' UTR of Staphylococcus aureus cidA.PloS one · 2019Article
- TheFrontiers in cellular and infection microbiology · 2019Article
- RNA Thermometers in Bacterial Pathogens.Microbiology spectrum · 2018Review
- Fever as an important resource for infectious diseases research.Intractable & rare diseases research · 2016Review
- Dynamic Proteome Response of Pseudomonas aeruginosa to Tobramycin Antibiotic Treatment.Molecular & cellular proteomics : MCP · 2015Article
- Mechanistic insights into temperature-dependent regulation of the simple cyanobacterial hsp17 RNA thermometer at base-pair resolution.Nucleic acids research · 2015Article
- Site-specific fluorescence dynamics in an RNA 'thermometer' reveals the role of ribosome binding in its temperature-sensitive switch function.Nucleic acids research · 2015Article
- Tet-Trap, a genetic approach to the identification of bacterial RNA thermometers: application to Pseudomonas aeruginosa.RNA (New York, N.Y.) · 2014Article
- The PqsR and RhlR transcriptional regulators determine the level of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa by producing two different pqsABCDE mRNA isoforms.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial small heat shock protein IbpA protects client proteins from aggregation. Due to redundancy in the cellular chaperone network, deletion of the ibpA gene often leads to only a mild or no phenotypic defect. In this study, we show that a Pseudomonas putida ibpA deletion mutant has a severe growth defect under heat stress conditions and reduced survival during recovery revealing a critical role of IbpA in heat tolerance. Transcription of the ibpA gene depends on the alternative heat shock sigma factor σ(32). Production of IbpA protein only at heat shock temperatures suggested additional translational control. We conducted a comprehensive structural and functional analysis of the 5' untranslated regions of the ibpA genes from P. putida and Pseudomonas aeruginosa. Both contain a ROSE-type RNA thermometer that is substantially shorter and simpler than previously reported ROSE elements. Comprised of two hairpin structures only, they inhibit translation at low temperature and permit translation initiation after a temperature upshift. Both elements regulate reporter gene expression in Escherichia coli and ribosome binding in vitro in a temperature-dependent manner. Structure probing revealed local melting of the second hairpin whereas the first hairpin remained unaffected. High sequence and structure conservation of pseudomonal ibpA untranslated regions and their ability to confer thermoregulation in vivo suggest that short ROSE-like thermometers are commonly used to control IbpA synthesis in Pseudomonas species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.