ArticlePloS one2013
Pharmacological characterization of a 5-HT1-type serotonin receptor in the red flour beetle, Tribolium castaneum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Rapid behavioural gregarization in the desert locust, Schistocerca gregaria entails synchronous changes in both activity and attraction to conspecifics.Journal of insect physiology · 2014Pooled it
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- Identification and Pharmacological Characterization of Two Serotonin Type 7 Receptor Isoforms fromInternational journal of molecular sciences · 2022Article
- Deletion of the Serotonin Receptor 7 Gene Changed the Development and Behavior of the Mosquito,Insects · 2022Article
- Characterization of a new type of neuronal 5-HT G- protein coupled receptor in the cestode nervous system.PloS one · 2021Article
- Involvement ofFrontiers in physiology · 2021Article
- Serotonergic modulation across sensory modalities.Journal of neurophysiology · 2020Review
- Serotonin in Animal Cognition and Behavior.International journal of molecular sciences · 2020Review
- Phenylethylamides derived from bacterial secondary metabolites specifically inhibit an insect serotonin receptor.Scientific reports · 2019Article
- Sex differences in aggression: Differential roles of 5-HT2, neuropeptide F and tachykinin.PloS one · 2019Article
- Parasitoid Jewel Wasp Mounts Multipronged Neurochemical Attack to Hijack a Host Brain.Molecular & cellular proteomics : MCP · 2019Article
- Cloning and functional characterization of the DA2 receptor gene in Chinese mitten crab (Eriocheir sinensis).PloS one · 2018Article
- Article
- Identification, functional characterization, and pharmacological profile of a serotonin type-2b receptor in the medically important insect, Rhodnius prolixus.Frontiers in neuroscience · 2015Article
- Mechanisms underlying dual effects of serotonin during development of Helisoma trivolvis (Mollusca).BMC developmental biology · 2014Article
- Signaling properties and pharmacological analysis of two sulfakinin receptors from the red flour beetle, Tribolium castaneum.PloS one · 2014Article
- Pharmacological modulation of aversive responsiveness in honey bees.Frontiers in behavioral neuroscience · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serotonin (5-hydroxytryptamine, 5-HT) is known for its key role in modulating diverse physiological processes and behaviors by binding various 5-HT receptors. However, a lack of pharmacological knowledge impedes studies on invertebrate 5-HT receptors. Moreover, pharmacological information is urgently needed in order to establish a reliable classification system for invertebrate 5-HT receptors. In this study we report on the molecular cloning and pharmacological characterization of a 5-HT1 receptor from the red flour beetle, Tribolium castaneum (Trica5-HT1). The Trica5-HT1 receptor encoding cDNA shows considerable sequence similarity with members of the 5-HT1 receptor class. Real time PCR showed high expression in the brain (without optic lobes) and the optic lobes, consistent with the role of 5-HT as neurotransmitter. Activation of Trica5-HT1 in mammalian cells decreased NKH-477-stimulated cyclic AMP levels in a dose-dependent manner, but did not influence intracellular Ca(2+) signaling. We studied the pharmacological profile of the 5-HT1 receptor and demonstrated that α-methylserotonin, 5-methoxytryptamine and 5-carboxamidotryptamine acted as agonists. Prazosin, methiothepin and methysergide were the most potent antagonists and showed competitive inhibition in presence of 5-HT. This study offers important information on a 5-HT1 receptor from T. castaneum facilitating functional research of 5-HT receptors in insects and other invertebrates. The pharmacological profiles may contribute to establish a reliable classification scheme for invertebrate 5-HT receptors.
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