ArticleAngewandte Chemie (International ed. in English)2013
Cobalt(III) as a stable and inert mediator ion between NTA and His6-tagged proteins.
Article in Angewandte Chemie (International ed. in English), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 101 citations in OpenAlex.
- Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.ACS applied nano materials · 2026Article
- Affinity-Based Copolymer Coating for Oriented Protein Immobilization in Biosensor Development.Biosensors · 2025Article
- A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.Bioconjugate chemistry · 2023Article
- High-Dose Exposure to Polymer-Coated Iron Oxide Nanoparticles Elicits Autophagy-Dependent Ferroptosis in Susceptible Cancer Cells.Nanomaterials (Basel, Switzerland) · 2023Article
- Review
- Injectable Liposome-based Supramolecular Hydrogels for the Programmable Release of Multiple Protein Drugs.Matter · 2022Article
- Stability of HA2 Prefusion Structure and pH-Induced Conformational Changes in the HA2 Domain of H3N2 Hemagglutinin.Biochemistry · 2021Article
- Dynamic Stereoselection of Peptide Helicates and Their Selective Labeling of DNA Replication Foci in Cells*.Angewandte Chemie (International ed. in English) · 2021Article
- Developing Eco-Friendly and Cost-Effective Porous Adsorbent for Carbon Dioxide Capture.Molecules (Basel, Switzerland) · 2021Article
- Kinetics of Histidine-Tagged Protein Association to Nickel-Decorated Liposome Surfaces.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Oriented Immobilization and Quantitative Analysis Simultaneously Realized in Sandwich Immunoassay via His-Tagged Nanobody.Molecules (Basel, Switzerland) · 2019Article
- Physical properties, ligand substitution reactions, and biological activity of Co(iii)-Schiff base complexes.Dalton transactions (Cambridge, England : 2003) · 2019Article
- Article
- Cyclotron production and radiochemical separation ofApplied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine · 2017Article
- Functionalization of cobalt porphyrin-phospholipid bilayers with his-tagged ligands and antigens.Nature chemistry · 2015Article
- A simple method to engineer a protein-derived redox cofactor for catalysis.Biochimica et biophysica acta · 2014Article
- Article
- T cell activation is determined by the number of presented antigens.Nano letters · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.