ArticleTransgenic research2013
Multigene engineering of starch biosynthesis in maize endosperm increases the total starch content and the proportion of amylose.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).Planta · 2023Article
- Genome-wide association analysis of kernel nutritional quality in two natural maize populations.Molecular breeding : new strategies in plant improvement · 2023Article
- Genetic Engineering of Starch Biosynthesis in Maize Seeds for Efficient Enzymatic Digestion of Starch during Bioethanol Production.International journal of molecular sciences · 2023Review
- Physicochemical properties and molecular mechanisms of different resistant starch subtypes in rice.Frontiers in plant science · 2023Article
- Effect of calcium and magnesium on starch synthesis in maize kernels and its physiological driving mechanism.Frontiers in plant science · 2023Article
- Genetic dissection of QTLs for starch content in four maize DH populations.Frontiers in plant science · 2022Article
- Changes of starch and sucrose content and related gene expression during the growth and development of Lanzhou lily bulb.PloS one · 2022Article
- Corn Starch: Quality and Quantity Improvement for Industrial Uses.Plants (Basel, Switzerland) · 2021Review
- Genetic basis of kernel starch content decoded in a maize multi-parent population.Plant biotechnology journal · 2021Article
- Genome-wide association studies of grain quality traits in maize.Scientific reports · 2021Article
- Flavonoids Modulate the Accumulation of Toxins FromFrontiers in plant science · 2021Article
- An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.Science advances · 2020Article
- Article
- Crop resistant starch and genetic improvement: a review of recent advances.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Review
- Identification of Quantitative Trait Loci and Candidate Genes for Maize Starch Granule Size through Association Mapping.Scientific reports · 2018Article
- The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology.Frontiers in plant science · 2018Review
- Functional Analysis of a Wheat AGPase Plastidial Small Subunit with a Truncated Transit Peptide.Molecules (Basel, Switzerland) · 2017Article
- Whole-Genome Analysis of Candidate genes Associated with Seed Size and Weight inFrontiers in plant science · 2017Article
- Article
- The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize (Zea mays spp. mays) is a staple crop for more than 900 million people. The seeds or kernels provide a rich source of calories because ~70% of the weight is carbohydrate, mostly in the form of starch. The content and composition of starch are complex traits controlled by many genes, offering multiple potential targets for intervention. We used a multigene engineering approach combining the overexpression of Bt2, Sh2, Sh1 and GbssIIa (to enhance the activity of sucrose synthase, AGPase and granule-bound starch synthase) with the suppression of SbeI and SbeIIb by RNA interference (to reduce the activity of starch branching enzyme). Maize plants expressing all six genes plus the selectable marker showed a 2.8-7.7% increase in the endosperm starch content and a 37.8-43.7% increase in the proportion of amylose, which was significant compared to untransformed control plants. We also observed improvements in other agronomic traits, such as a 20.1-34.7% increase in 100-grain weight, a 13.9-19.0% increase in ear weight, and larger kernels with a better appearance, presumably reflecting the modified starch structure within the kernels. Our results confirm that multigene engineering applied to the starch biosynthesis pathway can not only modulate the quality and quantity of starch but can also improve starch-dependent agronomic traits.
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