ArticleAmerican journal of physiology. Renal physiology2013
Attenuation of lithium-induced natriuresis and kaliuresis in P2Y₂ receptor knockout mice.
Article in American journal of physiology. Renal physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
- Purinergic signaling is enhanced in the absence of UT-A1 and UT-A3.Physiological reports · 2021Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- ATP release into ADPKD cysts via pannexin-1/P2X7 channels decreases ENaC activity.Biochemical and biophysical research communications · 2019Article
- Direct and indirect inhibition of the circadian clock protein Per1: effects on ENaC and blood pressure.American journal of physiology. Renal physiology · 2019Article
- Chronic lithium treatment induces novel patterns of pendrin localization and expression.American journal of physiology. Renal physiology · 2018Article
- Genetic Deletion of P2YFrontiers in physiology · 2018Article
- P2Y2R Signaling Is Involved in the Onset of Glomerulonephritis.Frontiers in immunology · 2018Article
- Prasugrel suppresses development of lithium-induced nephrogenic diabetes insipidus in mice.Purinergic signalling · 2017Article
- Regulation of Vascular and Renal Function by Metabolite Receptors.Annual review of physiology · 2016Review
- Clopidogrel attenuates lithium-induced alterations in renal water and sodium channels/transporters in mice.Purinergic signalling · 2015Article
- P2Y12 Receptor Localizes in the Renal Collecting Duct and Its Blockade Augments Arginine Vasopressin Action and Alleviates Nephrogenic Diabetes Insipidus.Journal of the American Society of Nephrology : JASN · 2015Article
- Impaired natriuretic response to high-NaCl diet plus aldosterone infusion in mice overexpressing human CD39, an ectonucleotidase (NTPDase1).American journal of physiology. Renal physiology · 2015Article
- Targeting renal purinergic signalling for the treatment of lithium-induced nephrogenic diabetes insipidus.Acta physiologica (Oxford, England) · 2015Review
- Article
- Cellular localization of adenine receptors in the rat kidney and their functional significance in the inner medullary collecting duct.American journal of physiology. Renal physiology · 2013Article
- P2Y2 receptor knock-out mice display normal NaCl absorption in medullary thick ascending limb.Frontiers in physiology · 2013Article
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Authors and funding
5 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Whole body knockout (KO) of the P2Y₂ receptor (P2Y₂R) results in enhanced vasopressin V2 receptor activity and increased renal Na⁺ conservation. We hypothesized that P2Y₂R KO mice would be less sensitive to lithium-induced natriuresis and kaliuresis due to attenuated downregulation of one or more of the major renal Na⁺ or K⁺ transporter/channel proteins. KO and wild-type (WT) mice were fed a control or lithium-added diet (40 mmol/kg food) for 14 days. Lithium-induced natriuresis and kaliuresis were significantly (~25%) attenuated in KO mice. The subunits of the epithelial Na⁺ channel (ENaC) were variably affected by lithium and genotype, but, overall, medullary levels were decreased substantially by lithium (15-60%) in both genotypes. In contrast, cortical, β-, and γ-ENaC were increased by lithium (~50%), but only in WT mice. Moreover, an assessment of ENaC activity by benzamil sensitivity suggested that lithium increased ENaC activity in WT mice but in not KO mice. In contrast, medullary levels of Na⁺-K⁺-2Cl⁻ cotransporter 2 and cortical levels of the renal outer medullary K⁺ channel were not downregulated by lithium and were significantly (15-76%) higher in KO mice under both dietary conditions. In addition, under control conditions, tissue osmolality of the inner medulla as well as furosemide sensitivity were significantly higher in KO mice versus WT mice. Therefore, we suggest that increased expression of these proteins, particularly in the control state, reduces Na⁺ delivery to the distal nephron and provides a buffer to attenuate collecting duct-mediated natriuresis and kaliuresis. Additional studies are warranted to explore the potential therapeutic benefits of purinergic antagonism.
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