ReviewExpert opinion on drug discovery2013
Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization.
Review in Expert opinion on drug discovery, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Synergistic Orchestration of Chlorogenic Acid Analogs in Sugarcane Molasses Polyphenols for Sucrase Inhibition: Insights into a Multilevel Inhibitory Mechanism.Foods (Basel, Switzerland) · 2026Article
- Deciphering the Function and Structure of PA1216 as an S-Adenosyl-l-Methionine Binding Protein Using Differential Scanning Fluorimetry and Circular Dichroism.Protein science : a publication of the Protein Society · 2026Article
- pH-induced changes in hydrophobicity and key amino acid exposure regulate myoglobin digestibility.Food chemistry: X · 2026Article
- Applications of Differential Scanning Fluorimetry and Related Technologies in Characterization of Protein-Ligand Interactions.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Aurora 2.0: A fluorogenic dye library for expanding the capability of protein-adaptive differential scanning fluorimetry (paDSF).SLAS discovery : advancing life sciences R & D · 2025Article
- Chemical Evolution of Aplithianine Class of Serine/Threonine Kinase Inhibitors.Journal of medicinal chemistry · 2025Article
- Thermal shift assay to identify ligands for bacterial sensor proteins.FEMS microbiology reviews · 2025Review
- Protein-adaptive differential scanning fluorimetry using conformationally responsive dyes.Nature biotechnology · 2025Article
- DSFworld: A flexible and precise tool to analyze differential scanning fluorimetry data.Protein science : a publication of the Protein Society · 2024Article
- Ligands of HMG-like dorsal switch protein 1 ofHeliyon · 2024Article
- Peptidyl nitroalkene inhibitors of main protease rationalized by computational and crystallographic investigations as antivirals against SARS-CoV-2.Communications chemistry · 2024Article
- Protocol for performing and optimizing differential scanning fluorimetry experiments.STAR protocols · 2023Article
- Thermal unfolding methods in drug discovery.Biophysics reviews · 2023Review
- Ion Mobility Mass Spectrometry (IM-MS) for Structural Biology: Insights Gained by Measuring Mass, Charge, and Collision Cross Section.Chemical reviews · 2023Review
- Screening of Buffers and Additives for Protein Stabilization by Thermal Shift Assay: A Practical Approach.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries.Frontiers in pharmacology · 2022Article
- An update of label-free protein target identification methods for natural active products.Theranostics · 2022Review
- Inhibition of Autophagy by a Small Molecule through Covalent Modification of the LC3 Protein.Angewandte Chemie (International ed. in English) · 2021Article
- Discovery of new non-pyrimidine scaffolds asJournal of enzyme inhibition and medicinal chemistry · 2021Article
- Synthesis and biological evaluation of thiazolidinedione derivatives with high ligand efficiency toJournal of enzyme inhibition and medicinal chemistry · 2021Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
introductionDespite tremendous advances in the application of biophysical methods in drug discovery, the preponderance of instruments and techniques still require sophisticated analyses by dedicated personnel and/or large amounts of frequently hard-to-produce proteins. A technique which carries the promise of simplicity and relatively low protein consumption is the differential scanning fluorometry (DSF). This technique monitors protein through the use of environmentally sensitive fluorescent dye, in a temperature-ramp regime by observing the gradual exposure to the solvent of otherwise buried hydrophobic faces of protein domains. AREAS COVERED: This review describes recent developments in the field of DSF. This article pays a particular emphasis on the advances published during the 2010 - 2013 period. EXPERT OPINION: There has been a significant diversification of DSF applications beyond initial small molecule discovery into areas such as protein therapeutic development, formulation studies and various mechanistic investigations. This serves as a further indication of the broad penetration of the technique. In the small molecule arena, DSF has expanded toward sophisticated co-dependency MOA tests, demonstrating the wealth of information which the technique can provide. Importantly, the first public deposition of a large screening dataset may enable the use of thermal stabilization data in refining in silico models for small molecule binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.