ArticleBioMed research international2013
Revealing the mechanism of in vitro wound healing properties of Citrus tamurana extract.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Emodin and aloe-emodin, two potential molecules in regulating cell migration of skin cells through the MAP kinase pathway and affecting Caenorhabditis elegans thermotolerance.BMC molecular and cell biology · 2023Article
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- Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis.Biomaterials science · 2021Article
- Modulation of Cellular Response to Different Parameters of the Rotating Magnetic Field (RMF)-An In Vitro Wound Healing Study.International journal of molecular sciences · 2021Article
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- In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration.Journal of visualized experiments : JoVE · 2019Article
- Buckwheat and buckwheat enriched products exert an anti-inflammatory effect on the myofibroblasts of colon CCD-18Co.Food & function · 2018Article
- Article
- Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing.Clinical oral investigations · 2015Article
- Exploration of alginate hydrogel/nano zinc oxide composite bandages for infected wounds.International journal of nanomedicine · 2015Article
- Citrus fruits as a treasure trove of active natural metabolites that potentially provide benefits for human health.Chemistry Central journal · 2015Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present investigation, we examined the effect of Hyuganatsu (Citrus tamurana) extract (HE) on skin fibroblast (TIG-119) proliferation and migration during in vitro wound healing. HE selectively inhibited proliferation of TIG-119 cells at higher concentration (>1.0 mg/mL); at lower concentrations (0.1, 0.25, 0.5, and 0.75 mg/mL), it exhibited linear and time-dependent cell proliferation. In vitro scratch wound healing studies showed that the HE also accelerated the migration of cells towards the wounded region. Cytometric analysis demonstrated that HE extract did not alter G1/0 and S phases of cell cycle in any concentration studied; however, G2/M phases of cell cycle were significantly (P < 0.05) accelerated at 0.75 mg/mL dose. RT-PCR and Western blotting analysis indicated that HE markedly overexpressed levels of Rac-1, Rho-A, and Cdc-42 mRNA and the respective proteins. Cyclin-dependent kinases (Cdk-1 and -2) gene expression activity was significantly (P < 0.05) increased, but protein content decreased during treatment with HE. The induction of Cdk-1 and -2 by HE was abolished by inhibitors, transcription (DRB), and translation (CHX), implying transcriptional regulation that required de novo protein synthesis.
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