ArticleBioMed research international2013
Evaluation of adverse effects of mutein forms of recombinant human interferon alpha-2b in female swiss webster mice.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 2 citations in OpenAlex.
- From Genetic Engineering to Preclinical Safety: A Study on Recombinant Human Interferons.International journal of molecular sciences · 2025Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeWe successfully developed recombinant human interferon alpha-2b (rhIFN- α 2b) and mutein forms through the site-directed mutagenesis technique. The mutein forms were developed by substituting cysteins at positions 2 and 99 with aspartic acids. The potential adverse effects of these rhIFN- α 2bs were assessed by acute and subchronic studies.
methodsIn the acute study, rhIFN- α 2bs were subcutaneously administered to mice at a single dose of 97.5 μ g/kg, 975 μ g/kg, and 9.75 mg/kg BW and were observed for 14 days. In the subchronic study, single dose of 1.95 μ g/kg and 19.5 μ g/kg, respectively, was given subcutaneously every 3 days for 45 days.
resultsNo death as well as abnormality in body weight, behavior, presentation of main organs, and value of plasma SGPT and SGOT was observed. Wild type and mutein rhIFN- α 2bs did not show significant adverse effects at dose up to 9.75 mg/kg BW. Administration of these rhIFN- α 2bs given repeatedly did not induce any adverse effect.
conclusionThese results suggest that our rhIFN- α 2bs are safe. However, further study is still needed to clarify the safety issue before use in clinical trial.
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