ArticlePloS one2013
Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
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- Scarless gene disruption enabled by a dual-plasmid knockout platform in a clinical infant-derivedFrontiers in microbiology · 2025Article
- An improved temperature-sensitive shuttle vector system for scarless gene deletion in human-gut-associatediScience · 2024Article
- Harnessing the endogenous Type I-C CRISPR-Cas system for genome editing inApplied and environmental microbiology · 2024Article
- Siderophore Synthesis Ability of the Nitrogen-Fixing Bacterium (NFB) GXGL-4A is Regulated at the Transcriptional Level by a Transcriptional Factor (trX) and an Aminomethyltransferase-Encoding Gene (amt).Current microbiology · 2022Article
- M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform.PLoS biology · 2022Article
- Comparative Population Genetics in the Human Gut Microbiome.Genome biology and evolution · 2022Article
- Molecular analysis of the replication functions of the bifidobacterial conjugative megaplasmid pMP7017.Microbial biotechnology · 2021Article
- Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure.Scientific reports · 2020Article
- Application of Recombinase-Based In Vivo Expression Technology toMicroorganisms · 2020Article
- Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates.AMB Express · 2019Article
- A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an ISApplied and environmental microbiology · 2018Article
- Bifidobacterium breve UCC2003 Employs Multiple Transcriptional Regulators To Control Metabolism of Particular Human Milk Oligosaccharides.Applied and environmental microbiology · 2018Article
- Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve.Nucleic acids research · 2018Article
- Global transcriptional landscape and promoter mapping of the gut commensal Bifidobacterium breve UCC2003.BMC genomics · 2017Article
- The essential genomic landscape of the commensal Bifidobacterium breve UCC2003.Scientific reports · 2017Article
- Mechanistic Study of Utilization of Water-Insoluble Saccharomyces cerevisiae Glucans by Bifidobacterium breve Strain JCM1192.Applied and environmental microbiology · 2017Article
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4 authors.
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Abstract
Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.
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