ArticlePLoS pathogens2013
Murinization of internalin extends its receptor repertoire, altering Listeria monocytogenes cell tropism and host responses.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- ComparingGut microbes · 2025Article
- Listeriosis.Nature reviews. Disease primers · 2025Review
- Bacterial aggregation facilitates internalin-mediated invasion ofFrontiers in cellular and infection microbiology · 2024Article
- Current methodologies available to evaluate the virulence potential amongFrontiers in microbiology · 2024Review
- Listeria motility increases the efficiency of epithelial invasion during intestinal infection.PLoS pathogens · 2022Article
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- Pathogenicity and virulence ofVirulence · 2021Article
- Human Placental Trophoblasts Infected byFrontiers in immunology · 2021Article
- Neurotropic Lineage III Strains of Listeria monocytogenes Disseminate to the Brain without Reaching High Titer in the Blood.mSphere · 2020Article
- Host Lipid Rafts as the Gates forFrontiers in immunology · 2020Review
- Surface Proteins ofMicrobiology spectrum · 2019Review
- Atypical HemolyticInfection and immunity · 2019Article
- Short-term consumption of a high-fat diet increases host susceptibility to Listeria monocytogenes infection.Microbiome · 2019Article
- Peyer's patch myeloid cells infection byThe Journal of experimental medicine · 2018Article
- The olfactory epithelium as a port of entry in neonatal neurolisteriosis.Nature communications · 2018Article
- Animal and Human Tissue Models of Vertical Listeria monocytogenes Transmission and Implications for Other Pregnancy-Associated Infections.Infection and immunity · 2018Review
- Vertical Transmission ofPathogens (Basel, Switzerland) · 2018Review
- A Comparison of Oral and Intravenous Mouse Models of Listeriosis.Pathogens (Basel, Switzerland) · 2018Review
- Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis.Nature reviews. Microbiology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes (Lm) is an invasive foodborne pathogen that leads to severe central nervous system and maternal-fetal infections. Lm ability to actively cross the intestinal barrier is one of its key pathogenic properties. Lm crosses the intestinal epithelium upon the interaction of its surface protein internalin (InlA) with its host receptor E-cadherin (Ecad). InlA-Ecad interaction is species-specific, does not occur in wild-type mice, but does in transgenic mice expressing human Ecad and knock-in mice expressing humanized mouse Ecad. To study listeriosis in wild-type mice, InlA has been "murinized" to interact with mouse Ecad. Here, we demonstrate that, unexpectedly, murinized InlA (InlA(m)) mediates not only Ecad-dependent internalization, but also N-cadherin-dependent internalization. Consequently, InlA(m)-expressing Lm targets not only goblet cells expressing luminally-accessible Ecad, as does Lm in humanized mice, but also targets villous M cells, which express luminally-accessible N-cadherin. This aberrant Lm portal of entry results in enhanced innate immune responses and intestinal barrier damage, both of which are not observed in wild-type Lm-infected humanized mice. Murinization of InlA therefore not only extends the host range of Lm, but also broadens its receptor repertoire, providing Lm with artifactual pathogenic properties. These results challenge the relevance of using InlA(m)-expressing Lm to study human listeriosis and in vivo host responses to this human pathogen.
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