ArticleInvestigative ophthalmology & visual science2013
Staphylococcus aureus corneal infections: effect of the Panton-Valentine leukocidin (PVL) and antibody to PVL on virulence and pathology.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Parameter-Dependent Effects of Rose Bengal Photodynamic Antimicrobial Therapy on MRSA Inhibition and Corneal Cytokine Expression.Translational vision science & technology · 2026Article
- Contribution of Leukocidin ED to the Pathogenesis of Staphylococcus aureus Endophthalmitis.Investigative ophthalmology & visual science · 2025Article
- First Report ofTransboundary and emerging diseases · 2024Article
- Article
- Staphylococcal Enterotoxins Promote Virulence in Bacterial Keratitis.Investigative ophthalmology & visual science · 2023Article
- Review
- The protective effect of URP20 on ocular Staphylococcus aureus and Escherichia coli infection in rats.BMC ophthalmology · 2022Article
- Article
- Virulence Genes of Staphylococcus aureus Associated With Keratitis, Conjunctivitis, and Contact Lens-Associated Inflammation.Translational vision science & technology · 2022Article
- Development of topical chitosan/ β-glycerophosphate-based hydrogel loaded with levofloxacin in the treatment of keratitis: AnHeliyon · 2022Article
- Evaluation of the role of staphylococci in the pathomechanism of conjunctivitis.International ophthalmology · 2021Article
- Review
- An Eye onToxins · 2019Review
- Molecular epidemiology and resistance profiles among healthcare- and community-associatedInfection and drug resistance · 2019Article
- Article
- Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis.Investigative ophthalmology & visual science · 2018Article
- The Pathogenesis of Staphylococcus aureus Eye Infections.Pathogens (Basel, Switzerland) · 2018Review
- Ocular bacterial infections at Quiha Ophthalmic Hospital, Northern Ethiopia: an evaluation according to the risk factors and the antimicrobial susceptibility of bacterial isolates.BMC infectious diseases · 2017Article
- A Mouse Model for Ocular Surface Staphylococcus aureus Infection.Current protocols in mouse biology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
purposeCommunity-associated methicillin-resistant Staphylococcus aureus strains expressing Panton-Valentine leukocidin (PVL) are associated with severe skin and soft tissue infections, necrotizing pneumonia, and eye infections. We determined PVL's toxicity on infected mouse and cultured human corneal epithelial cells and the role of PVL and antibody to PVL in pathogenesis of murine keratitis.
methodsCytotoxicity on corneas and corneal epithelial cells was evaluated by LDH assays. Scratched corneas of female A/J mice were inoculated with approximately 10⁷ CFU/eye of either WT S. aureus, isogenic ΔPVL, or strains overproducing PVL. Antibodies to PVL or control sera were topically applied to infected corneas 0, 24, and 32 hours postinfection, corneas scored for pathology and tissue levels of S. aureus were determined.
resultsPVL expression augmented the cytotoxicity of S. aureus on infected mouse corneas and human cultured corneal epithelial cells. Variable effects on leukocyte recruitment, pathogenesis, and immunity were obtained in the in vivo studies. Inactivation of PVL in USA300 strains caused reduced pathology and bacterial counts. Results were variable when comparing WT and ΔPVL USA400 strains, while USA400 strains overproducing PVL caused increased bacterial burdens. Topical treatment with polyclonal antibody to PVL yielded significant reductions in corneal pathology and bacterial CFU in corneas infected with USA300 strains, whereas effects were inconsistent in eyes infected with USA400 strains.
conclusionsPVL enhanced the virulence of a subset of MRSA strains in a keratitis model. Coupled with a variable effect of antibody treatment, it appears that PVL plays an inconsistent role in pathogenesis and immunity to S. aureus corneal infection.
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